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Vol 40, No 3 (2023)

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Review Articles

Metabolic Plasticity of a Developing and Aging Brain

Salmina A.B.

Abstract

Brain plasticity is a fundamental phenomenon based on various types of intercellular interactions (synaptic activity, neuritogenesis, synaptogenesis and elimination of synapses, neuron-glia interactions), development, differentiation, migration of newly-born cells and cell death (neurogenesis/gliogenesis and neuronal or glial cell death, angiogenesis and regression of cerebral microvessels), adaptation of tissue metabolism to changing environmental conditions. In this review, we discuss our own data and available literature in the context of regulation of certain types of energy metabolism (glycolysis, mitochondrial respiration) in neuronal, glial, and endothelial cells, the signaling functions of metabolites in nervous tissue, the mechanisms of establishment of cerebral insulin resistance, pseudohypoxia and associated neuroinflammation in brain pathology, as well as some prospects for detecting novel molecular markers of pathobiochemical processes associated with impaired metabolic plasticity in the developing and aging brain.

Nejrohimiâ. 2023;40(3):197-210
pages 197-210 views

Markers of Neurodegeneration in Parkinson’s Disease

Nikitina M.A., Alifirova V.M., Borodina S.O., Koroleva E.S.

Abstract

This review describes the role of peripheral blood biomarkers involved in neurodegeneration and neuroregeneration in Parkinson’s disease: BDNF, Cathepsin D, NSAM, myeloperoxidase, plasminogen activator inhibitor-1 (PAI-1), platelet-derived growth factor (PDGF), regulated upon activation, normal T cell expressed and secreted (RANTES) and intercellular adhesion molecules (sICAM-1). These biomarkers are important indicators of biological processes and perspective for early diagnosis, prognosis of the disease and the development of new possibilities in modifying therapy for Parkinson’s disease as they are associated with neuroprotective and neurotrophic systems.

Nejrohimiâ. 2023;40(3):211-222
pages 211-222 views

Experimental Articles

Effect of In Vitro Culture and Embryo Transfer on Neuronal Density and Neurogenesis in the Brain of C57BL/6J Mice

Brusentsev E.Y., Igonina T.N., Rozhkova I.N., Okotrub S.V., Lebedeva D.A., Vladimirova E.V., Kozeneva V.S., Amstislavsky S.Y.

Abstract

The current research is aimed to determine the long-term effects of the in vitro culture (IVC) and embryo transfer (ET) on the neonatal offspring development, as well as on the adult hippocampal neuronal densities, as well hippocampal neurogenesis in С57BL/6J mice. Offspring of naturally born C57BL/6J mice (C57BL group) were compared with C57BL/6J mice born as a result of the IVC combined with ET to C57BL/6J recipient females (ET-C57BL group). At age of 3 mo., no group differences were observed in the body weight and brain-to-body ratio, although sex differences in these variables were observed. The offspring of both sexes born after IVC-ET exhibited the lower level of neurogenesis in the dentate gyrus (DG) of the hippocampus as compared to the control C57BL group. To conclude, IVC and ET exerted no major effects on body and brain weight in offspring, but affected hippocampal neurogenesis in the adult offspring of both sexes. Besides, the number of pyramidal neurons in the CA3 area of hippocampus was lower in female offspring of ET-C57BL group.

Nejrohimiâ. 2023;40(3):223-233
pages 223-233 views

Repeated Experience of Aggression Changes Gene Expression in Hypothalamus in Male Mice of Two Strains

Sapronova А.А., Kisaretovа P.E., Salman R., Bondar N.P.

Abstract

Repeated positive fighting experience can lead to changes in the neurophysiology and behavior of animals and to the formation of pathological aggression. Mechanisms of adaptation to repeated aggression are thought to be controlled through hypothalamus and HPA axis. In this work, we studied the effect of repeated (30 days) experience of aggression on gene expression in the hypothalamus in male mice of two strains – C57BL/6J and CD1. We measured expression levels of the HPA axis genes (Crh, Crhr1, Crhbp, Fkbp5, Nr3c1), as well as early response gene (Fos) and dopamine receptor D1 gene (Drd1). Repeated experience of aggression led to prolongation of Fos activation in C57BL/6J, although the expression of other studied genes remained unchanged. CD1 aggressors are characterized by decreased expression level of Crhr1 and Crhbp genes, whose products inhibit the secretion of corticotropin-releasing hormone. Most of the studied genes showed strain variation in gene expression that is probably associated with different levels of locomotor activity and anxiety in mice of these strains. The basal expression level of Crh gene was higher in C57BL/6J mice, while Crhr1, Crhbp, Fkbp5, Fos, and Drd1 genes were higher in CD1 mice. Thus, the repeated experience of aggression leads to gene expression changes in the hypothalamus of male mice, that depend on the genetic background and related psychophysiological features of individuals.

Nejrohimiâ. 2023;40(3):234-244
pages 234-244 views

JNK (c-Jun N-Terminal Kinase) Inhibitor IQ-1S Suppresses Premature Aging of OXYS Rat Brain

Zhdankina A.A., Osipenko A.N., Tikhonov D.I., Logvinov S.V., Plotnikov M.B., Khlebnikov A.I., Kolosova N.G.

Abstract

According to the international organization Alzheimer’s Disease International (ADI), about 50 million people in the world suffer from Alzheimer’s disease (AD). However, there are no effective methods for preventing and slowing down the progression of AD. Inhibition of the c-Jun N-terminal kinase (JNK) signaling pathway is being discussed as an alternative way to prevent the development of AD and other neurodegenerative diseases. In the present study, we evaluated the ability of a recently synthesized selective JNK3 inhibitor, 11H‑indeno[1,2-b]quinoxalin-11-on oxime sodium (IQ-1S), to suppress neurodegenerative processes in OXYS rats at an early stage of development of signs of AD at the age of 4, 5 to 6 months. Treatment with IQ-1S (50 mg/kg intragastrically) led to the suppression of the development of neurodegenerative processes in the cerebral cortex of OXYS rats: an increase in the proportion of unchanged neurons, a decrease in the proportion of neurons with signs of destruction and irreversible damage, and a normalization of the glioneuronal index, which was facilitated by a decrease in the severity of hyperviscosity syndrome blood in OXYS rats. The use of the JNK3 inhibitor IQ-1S may be a promising strategy for the prevention of early neurodegenerative disorders and, possibly, the treatment of AD.

Nejrohimiâ. 2023;40(3):245-256
pages 245-256 views

Differences in Changes in the Glutamate/GABA System Activity in the Rat Retina during Aging and the Development of Retinopathy at Nighttime and Daytime

Telegina D.V., Antonenko A.K., Kolosova N.G.

Abstract

Age is a leading risk factor for the development of age-related macular degeneration (AMD), which is the cause of vision loss in elderly. There are no effective methods of therapy for this complex neurodegenerative disease due to the incomplete knowledge of its etiology and pathogenesis. It is assumed that development of aging imbalance of neurotransmitter systems (glutamate, GABA) in the retina, their desynchronosis, may be a precondition for the development progression of AMD. Information about their state in the retina during aging, and especially development of AMD, is extremely limited. We previously assessed age-related changes in the daytime glutamate/GABA system in the retinas of Wistar and senescence-accelerated OXYS rats that develop AMD-like pathology. Here, we evaluated aging changes in the synthesis and degradation enzymes, receptor subunits, and transporters of these neurotransmitters at nighttime and compared them with detected earlier in the daytime. Differences in age-related changes in the expression of the components of the glutamate and GABAergic systems at night and daytime were revealed during “healthy” aging in Wistar rats and during premature aging in OXYS rats. This may be due to disruption of the circadian rhythm. It is established that the progression of AMD-like retinopathy in OXYS rats occurs against the background of changes in the glutamatergic system at the nighttime (increased of glutamine synthetase, NMDARr1 level and decreased GLAST level), which facilitate the retinal neurodegenerative changes. Pronounced changes in the GABAergic system, which could make a significant contribution to the development of the pathological process were not identified.

Nejrohimiâ. 2023;40(3):257-264
pages 257-264 views

Rapid Changes in the Expression of Active Caspase-3 and Glucocorticoid Receptors in Striatum Cells Induced by Neuroinflammation

Bulygina V.V., Shishkina G.T., Lanshakov D.A., Kalinina T.S., Komysheva N.P., Drozd U.S., Suhareva E.V., Dygalo N.N.

Abstract

Activation of microglia, resident immune cells of the central nervous system, plays a key role in the pathogenesis of neurological disorders induced by infections, as well as traumatic and ischemic events. Understanding the responses of brain cells, primarily microglial cells, to damaging effects can help overcome their pathological consequences. In this work, we analyzed the cellular effects of bacterial lipopolysaccharide (LPS), which is widely used as a pro-inflammatory stimulus. The injection of LPS into the area of right striatum of rats caused a pronounced neurological deficit in a day, which was accompanied by an increase in the number of microglial cells, an increase in the density of glucocorticoid receptors (GR) and their translocation into the nuclei of cells co-expressing the executive protease of apoptosis, active caspase-3 and GR, in the area of LPS injection. The results indicate acute changes in the activity of microglial cells, as well as in the expression and functional activity of GR in response to bacterial endotoxin. Further elucidation of the functional role of active caspase-3 and GR in microglial cells under conditions of pro-inflammatory activation may help identify targets for alleviating the symptoms of a neurological disorder.

Nejrohimiâ. 2023;40(3):265-272
pages 265-272 views

Dorsal Raphe Nucleus Serotonergic Neurons Activity Is Necessary for the Manifestation of the Antidepressant Effect of Ketamine

Drozd U.S., Lanshakov D.A., Dygalo N.N.

Abstract

Mechanisms of the ketamine antidepressant effects observed in humans and laboratory animals are not fully understood. To further clarify the role of the brain serotonergic (5-HT) activity in the drug antidepressant action, optogenetic inhibition of 5-HT neurons in the rat dorsal raphe nucleus (DRN) was applied. In control animals, a subanesthetic dose of ketamine alleviated their depressive-like behavior in the tail suspension test. Inhibition of 5-HT neurons abolished the drug effect and moreover, a sedative response to ketamine was found under these conditions. Furthermore, optogenetic suppression of the activity of 5-HT neurons prevented the increase in c-Fos expression induced by ketamine both in light-sensitive neurons and in other DRN neurons. The data emphasize the key role of 5-HT neuron activity in the rapid ketamine antidepressant effect.

Nejrohimiâ. 2023;40(3):273-280
pages 273-280 views

Effect of Subchronic Alcoholization on the Behavior and Monoaminergic Systems of the Brain of Mice with a Predisposition to Depression-Like Behavior

Bazovkina D.V., Kondaurova E.M., Adonina S.N., Bazhenova E.Y., Kulikov A.V.

Abstract

Depressive disorders and alcohol dependence are among the most common psychopathologies. It is known that disorders in the serotonergic and dopaminergic brain systems functioning lie in the pathogenesis of alcoholism and affective disorders. In this work, we studied the effects of prolonged administration of ethanol (1.5 g/kg, 20%, 10 days, i.p.) on behavior, functional activity of 5-HT1A and 5-HT2A receptors and expression of genes encoding serotonin (Htr1a, Htr2a) and dopamine (Drd1, Drd2) receptors in brain structures in mice of ASC strain (with the genetic predisposition to depressive-like behavior) and mice of the parental (“non-depressive”) CBA strain. It has been shown that alcoholization leads to an increase in motor activity in animals of both lines and an increase in the level of exploratory behavior in ASC mice. No significant effect of ethanol on social and depression-like behavior was found. The functional activities of 5-HT1A and 5-HT2A receptors (determined by the response to the administration of corresponding receptor agonists) were reduced by ethanol only in ASC animals. A decrease in 5-HT2A receptor gene expression was found in the frontal cortex of CBA mice treated with alcohol. At the same time, ethanol led to an increase in the mRNA levels of the 5-HT1A receptor gene in the striatum and the DRD1 receptor gene in the hypothalamus, as well as a decrease in the expression of the DRD2 receptor gene in the hippocampus of ASC mice. Thus, changes in the serotonergic and dopaminergic brain systems induced by chronic ethanol were more significant in ASC mice with a genetic predisposition to depression-like behavior.

Nejrohimiâ. 2023;40(3):281-291
pages 281-291 views

Clinical Neurochemistry

The Role of TNF-α, TNFRSF1A, and CD40 Genes Polymorfisms in Multiple Sclerosis in Tomsk Region

Titova M.A., Alifirova V.M., Musina N.F., Nikolaeva T.N.

Abstract

We studied the role of polymorphisms rs1800629 of the TNF-α gene; rs4149584 of the TNFRSF1A gene; rs6074022, rs1883832, rs1535045, rs11086996 of the CD40 gene in the onset, clinical course and response to treatment in multiple sclerosis (MS) in a group of 152 patients, living in Tomsk region. 707 volunteers without autoimmune diseases and pathology of the nervous system were included in control group. The allele C of the rs6074022 polymorphism of CD40 gene was associated with the risk of MS and contributed to the high rate of disease progression. The T allele of the rs6074022 polymorphism of CD40 gene showed a significant association with the average rate of disease progression, and the GA genotype of rs1800629 polymorphism of TNF-α gene was associated with a higher frequency of MS exacerbations. Other polymorphisms did not demonstrate an association with both the risk of disease, the clinical features and response to treatment.

Nejrohimiâ. 2023;40(3):292-298
pages 292-298 views

Features of Changes in Exogenous Heat Shock Proteins (HSP 27 and HSP 70) in Patients with Occupational Nervous System Pathology

Bodienkova G.M., Boklazhenko E.V.

Abstract

The aim of the study was to identify and compare changes in serum concentrations of heat shock proteins (HSP 27 and HSP 27) in patients with occupational nervous system pathology induced by exposure to physical and chemical factors. Patients with sensorineural hearing loss (SHL), chronic mercury intoxication (CMI), vibration disease (VD) formed both under the influence of local vibration and combined exposure to general and local vibration were examined. As a result of comparative evaluation and analysis of serum concentrations of heat shock proteins, peculiarities in their change were revealed. In individuals with SHL and VD due to local vibration exposure, a decrease in serum HSP 70 concentration was recorded, and in patients with CMI and VD due to combined local and general vibration exposure, high HSP 27 concentrations were recorded. The examination of patients in the linked sample at 3 years showed the absence of statistically significant differences and the preservation of changes in the content of exogenous HSPs, which confirms the progressive course of these diseases. The conjugation of the number of T-lymphocyte and B-cells subpopulations with the change in serum HSP concentrations was established, which confirms the role of HSP 27 and 70 in the regulation of the immune response in VD and allows them to be regarded as markers of cellular and tissue damage in the chronic course of diseases.

Nejrohimiâ. 2023;40(3):299-304
pages 299-304 views