Investigation of EpsA, OmpA, and Bap Genes among MDR and XDR Acinetobacter baumannii Isolates in Khorramabad, Iran


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Background:Acinetobacter baumannii is an opportunistic hospital pathogen with high antibiotic resistance, and the ability to produce biofilm. This study aimed to investigate epsA, ompA, and bap genes involved in biofilm formation in MDR and XDR clinical isolates of Acinetobacter baumannii in Khorramabad, Iran.

Methods:In this study, 79 A. baumannii isolates were collected from various samples of the patients admitted to tertiary hospitals in Khorramabad city, Iran, between January and August 2019. After performing the semi-quantitative evaluation of biofilm production by microtiter plate assay, screening of isolates carrying epsA, ompA, and bap genes was done by PCR method. Finally, statistical analyses were conducted using SPSS 22.

Results:Among 79 A.baumannii isolates, 52% XDR, 40% MDR, and 16% non-XDRMDR isolates were found to be biofilm producers. All XDR and 94% MDR isolates had ompA and epsA genes, and bap genes were detected among > 80% of these isolates. Moreover, the presence of biofilm-related genes and biofilm production among non-XDRMDR isolates were less than among resistant isolates (p≤ 0.01).

Conclusion:Based on the results, biofilm production and simultaneous presence of epsA, ompA, and bap genes among MDR, and XDR A. baumannii isolates have been found to be significantly more than non-XDR-MDR isolates.

Sobre autores

Iman Poladi

School of Medicine, Lorestan University of Medical Science

Email: info@benthamscience.net

Pegah Shakib

Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences

Email: info@benthamscience.net

Shahnaz Halimi

Department of Microbiology, School of Medicine, Tehran University of Medical Science

Email: info@benthamscience.net

Somayeh Delfani

Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences

Email: info@benthamscience.net

Farzad Zadeh

Nutritional Health Research Center, Department of Biostatistics and Epidemiology, School of Health and Nutrition, Lorestan University of Medical Sciences

Email: info@benthamscience.net

Faranak Rezaei

Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences

Autor responsável pela correspondência
Email: info@benthamscience.net

Bibliografia

  1. Lee CR, Lee JH, Park M, et al. Biology of Acinetobacter baumannii: Pathogenesis, antibiotic resistance mechanisms, and prospective treatment options. Front Cell Infect Microbiol 2017; 7: 55. doi: 10.3389/fcimb.2017.00055 PMID: 28348979
  2. Asif M, Alvi IA, Rehman SU. Insight into Acinetobacter baumannii: Pathogenesis, global resistance, mechanisms of resistance, treatment options, and alternative modalities. Infect Drug Resist 2018; 11: 1249-60. doi: 10.2147/IDR.S166750 PMID: 30174448
  3. Eijkelkamp BA, Stroeher UH, Hassan KA, Paulsen IT, Brown MH. Comparative analysis of surface-exposed virulence factors of Acinetobacter baumannii. BMC Genomics 2014; 15(1): 1020. doi: 10.1186/1471-2164-15-1020 PMID: 25422040
  4. Skariyachan S, Taskeen N, Ganta M, Venkata Krishna B. Recent perspectives on the virulent factors and treatment options for multidrug-resistant Acinetobacter baumannii. Crit Rev Microbiol 2019; 45(3): 315-33. doi: 10.1080/1040841X.2019.1600472 PMID: 31012772
  5. Kyriakidis I, Vasileiou E, Pana ZD, Tragiannidis A. Acinetobacter baumannii antibiotic resistance mechanisms. Pathogens 2021; 10(3): 373. doi: 10.3390/pathogens10030373 PMID: 33808905
  6. Vrancianu CO, Gheorghe I, Czobor IB, Chifiriuc MC. Antibiotic resistance profiles, molecular mechanisms and innovative treatment strategies of acinetobacter baumannii. Microorganisms 2020; 8(6): 935. doi: 10.3390/microorganisms8060935 PMID: 32575913
  7. Eze E, Chenia H, El Zowalaty M. Acinetobacter baumannii biofilms: effects of physicochemical factors, virulence, antibiotic resistance determinants, gene regulation, and future antimicrobial treatments. Infect Drug Resist 2018; 11: 2277-99. doi: 10.2147/IDR.S169894 PMID: 30532562
  8. Qi L, Li H, Zhang C, et al. Relationship between antibiotic resistance, biofilm formation, and biofilm-specific resistance in Acinetobacter baumannii. Front Microbiol 2016; 7: 483. doi: 10.3389/fmicb.2016.00483 PMID: 27148178
  9. Mancilla-Rojano J, Castro-Jaimes S, Ochoa SA, et al. Whole-genome sequences of five Acinetobacter baumannii strains from a child with leukemia M2. Front Microbiol 2019; 10: 132. doi: 10.3389/fmicb.2019.00132 PMID: 30787915
  10. Fattahian Y, Rasooli I, Mousavi Gargari SL, Rahbar MR, Darvish Alipour Astaneh S, Amani J. Protection against Acinetobacter baumannii infection via its functional deprivation of biofilm associated protein (Bap). Microb Pathog 2011; 51(6): 402-6. doi: 10.1016/j.micpath.2011.09.004 PMID: 21946278
  11. Ghasemi E, Ghalavand Z, Goudarzi H, et al. Phenotypic and genotypic investigation of biofilm formation in clinical and environmental isolates of Acinetobacter baumannii. Arch Clin Infect Dis 2018; 13(4): e12914. doi: 10.5812/archcid.12914
  12. Joshua AA, Girija ASS, Ganesh PS, Priyadharsini JV. Distribution of Biofilm-associated Genes among Acinetobacter baumannii by in silico PCR. J Pharm Res Int 2021; 33(58A): 140-9. doi: 10.9734/jpri/2021/v33i58A34099
  13. Donadu MG, Mazzarello V, Cappuccinelli P, et al. Relationship between the biofilm-forming capacity and antimicrobial resistance in clinical Acinetobacter baumannii isolates: Results from a laboratory-based in vitro study. Microorganisms 2021; 9(11): 2384. doi: 10.3390/microorganisms9112384 PMID: 34835509
  14. Gedefie A, Demsiss W, Belete MA, et al. Acinetobacter baumannii biofilm formation and its role in disease pathogenesis: A review. Infect Drug Resist 2021; 14: 3711-9. doi: 10.2147/IDR.S332051 PMID: 34531666
  15. Babaie Z, Delfani S, Rezaei F, Norolahi F, Mahdian S, Shakib P. Molecular detection of carbapenem resistance in Acinetobacter baumannii isolated from patients in khorramabad city, Iran. Infect Disord Drug Targets 2020; 20(4): 543-9. doi: 10.2174/1871526519666190517124314 PMID: 31099322
  16. Girija As S, Priyadharsini JV. CLSI based antibiogram profile and the detection of MDR and XDR strains of Acinetobacter baumannii isolated from urine samples. Med J Islam Repub Iran 2019; 33: 3. doi: 10.47176/mjiri.33.3 PMID: 31086782
  17. Magiorakos AP, Srinivasan A, Carey RB, et al. Multidrug-resistant, extensively drug-resistant and pandrug-resistant bacteria: An international expert proposal for interim standard definitions for acquired resistance. Clin Microbiol Infect 2012; 18(3): 268-81. doi: 10.1111/j.1469-0691.2011.03570.x PMID: 21793988
  18. Zheljazkov VD, Cantrell CL, Tekwani B, Khan SI. Content, composition, and bioactivity of the essential oils of three basil genotypes as a function of harvesting. J Agric Food Chem 2008; 56(2): 380-5. doi: 10.1021/jf0725629 PMID: 18095647
  19. Zeighami H, Valadkhani F, Shapouri R, Samadi E, Haghi F. Virulence characteristics of multidrug resistant biofilm forming Acinetobacter baumannii isolated from intensive care unit patients. BMC Infect Dis 2019; 19(1): 629. doi: 10.1186/s12879-019-4272-0 PMID: 31315572
  20. Kengkla K, Kongpakwattana K, Saokaew S, Apisarnthanarak A, Chaiyakunapruk N. Comparative efficacy and safety of treatment options for MDR and XDR Acinetobacter baumannii infections: A systematic review and network meta-analysis. J Antimicrob Chemother 2018; 73(1): 22-32. doi: 10.1093/jac/dkx368 PMID: 29069421
  21. Ibrahim S, Al-Saryi N, Al-Kadmy IMS, Aziz SN. Multidrug-resistant Acinetobacter baumannii as an emerging concern in hospitals. Mol Biol Rep 2021; 48(10): 6987-98. doi: 10.1007/s11033-021-06690-6 PMID: 34460060
  22. Babapour E, Haddadi A, Mirnejad R, Angaji S-A, Amirmozafari N. Biofilm formation in clinical isolates of nosocomial Acinetobacter baumannii and its relationship with multidrug resistance. Infect Drug Resist 2016; 6(6): 528-33.
  23. Haghighifar E, Nasr-Esfahani B, Fazeli H. Determination of biofilm formation ability and antibiotic resistance of Acinetobacter baumannii strains isolated from patients with burn wound infection. Majallah-i Danishkadah-i Pizishki-i Isfahan 2019; 37(552): 1280-5.
  24. Azizi O, Shahcheraghi F, Salimizand H, et al. Molecular analysis and expression of bap gene in biofilm-forming multi-drug-resistant Acinetobacter baumannii. Rep Biochem Mol Biol 2016; 5(1): 62-72. PMID: 28070537
  25. Thummeepak R, Kongthai P, Leungtongkam U, Sitthisak S. Distribution of virulence genes involved in biofilm formation in multi-drug resistant Acinetobacter baumannii clinical isolates. Int Microbiol 2016; 19(2): 121-9. PMID: 27845499
  26. Gallant CV, Daniels C, Leung JM, et al. Common β‐lactamases inhibit bacterial biofilm formation. Mol Microbiol 2005; 58(4): 1012-24. doi: 10.1111/j.1365-2958.2005.04892.x PMID: 16262787
  27. Gaddy JA, Tomaras AP, Actis LA. The Acinetobacter baumannii 19606 OmpA protein plays a role in biofilm formation on abiotic surfaces and in the interaction of this pathogen with eukaryotic cells. Infect Immun 2009; 77(8): 3150-60. doi: 10.1128/IAI.00096-09 PMID: 19470746

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