MLE/DHX9 HELICASE ACTIVITY IS REQUIRED TO REGULATE THE EXPRESSION OF A NUMBER OF TISSUE-SPECIFIC GENES ON CHROMOSOME 4 IN DROSOPHILA MELANOGASTER

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Аннотация

DHX9 helicase and its ortholog MLE in D. melanogaster participate in different stages of gene expression. Both helicases are important for the formation and functioning of the nervous system in humans and D. melanogaster, respectively. However, the role of helicase activity of DHX9 and MLE in the regulation of gene expression has been poorly studied, and the existing data are quite contradictory. This work is devoted to the study of the role of helicase activity of MLE in the regulation of gene expression in D. melanogaster. On chromosome 4 of D. melanogaster, in locus 102F, a site of intense MLE binding was found. It was shown that MLE is a co-activator of expression of the Dyrk3, Toy, Sox102F, Shaven and Fuss genes located in this locus. For this, the helicase activity of MLE is required. Genes whose expression depends on MLE are expressed at a high level in the nervous system of D. melanogaster and are necessary for its proper development. The obtained data contribute to the study of potentially evolutionarily conserved functions of MLE.

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Авторлар туралы

I. Zolin

Engelhardt Institute of Molecular Biology, Russian Academy of Sciences

Moscow, Russian Federation

A. Grigel

Engelhardt Institute of Molecular Biology, Russian Academy of Sciences

Moscow, Russian Federation

S. Georgieva

Engelhardt Institute of Molecular Biology, Russian Academy of Sciences

Academician of the RAS Moscow, Russian Federation

A. Krasnov

Institute of Gene Biology, Russian Academy of Sciences

Moscow, Russian Federation

J. Nikolenko

Engelhardt Institute of Molecular Biology, Russian Academy of Sciences

Email: julia.v.nikolenko@gmail.com
Moscow, Russian Federation

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