Comparison of the Diagnostic Performance of Antigen B Purified from Sheep Hydatid Cyst Fluid (HCF) with Commercial ELISA Kit


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Abstract

Introduction::Cystic echinococcosis (CE) is a zoonotic parasitic disease caused by the metacestode of Echinococcus granulosus. CE is a health problem in Middle Eastern countries, such as Iran. The purpose of this study was to purify subunit 8 KDa antigen B from crude sheep hydatid cyst fluid (HCF) and compare its sensitivity and specificity with a commercial human ELISA kit (PT-Hydatid-96).

Methods::28 sera samples were collected from hydatid cyst patients who had surgery for a hydatid cyst and had their disease confirmed by pathology after the surgery. Furthermore, 35 samples of healthy individuals with no history of hydatid cysts were collected, as were nine serum samples from parasite-infected non-CE patients. HCF was obtained from sheep fertile cysts at a Sari slaughterhouse and used as an antigen. In an indirect ELISA test, the B antigen was employed, and the results were compared to those from a commercial ELISA kit.

Results::The results of this study were analyzed using the Kappa test. The commercial ELISA kit showed 17 cases (23.6%) positive, 44 cases (61.1%) negative, and 11 cases (15.3%) borderline. B antigen showed that 18 (25%), 43 (59.7 %), and 11 (15.3%) were positive, negative, and borderline, respectively. One sample (1.4% of 72 total samples) of 35 serum samples from healthy individuals was positive using B antigen-based ELISA. In addition, all nine serum samples from parasite-infected non-CE patients were negative for both tests. The sensitivity and specificity of the commercial ELISA kit have been evaluated at 60.7% and 100%, respectively. For B antigenbased ELISA, these values are 64.3 and 97.7%, respectively.

Conclusion::Antigen B produced from hydatid cyst fluid is a promising option for serological identification of hydatid cysts in both infected and healthy individuals. In an indirect ELISA test, hydatid fluid antigen could be used as a precise source of detection.

About the authors

Tahereh Galeh

Department of Parasitology, School of Medicine, Mazandaran University of Medical Sciences

Email: info@benthamscience.net

Mahdi Fakhar

Department of Parasitology, School of Medicine, Mazandaran University of Medical Sciences

Email: info@benthamscience.net

Majid Harandi

Department of Parasitology, School of Medicine, Kerman University of Medical Sciences

Email: info@benthamscience.net

Shirzad Gholami

Department of Parasitology, School of Medicine, Mazandaran University of Medical Sciences

Author for correspondence.
Email: info@benthamscience.net

Fatemeh Darzi

Department of Parasitology, School of Medicine, Mazandaran University of Medical Sciences

Email: info@benthamscience.net

Hossein Asgarian-Omran

Department of Immunology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran, Mazandaran University of Medical Sciences

Email: info@benthamscience.net

Shahabeddin Sarvi

Department of Parasitology, School of Medicine, Mazandaran University of Medical Sciences

Email: info@benthamscience.net

Reza Valadan

Department of Immunology, School of Medicine, Mazandaran University of Medical Sciences

Email: info@benthamscience.net

Maryam Hataminejad

Department of Parasitology, School of Medicine, Mazandaran University of Medical Sciences

Email: info@benthamscience.net

Sabah Mayahi

Doctor of Molecular Medicine, School of Medicine, Mazandaran University of Medical Sciences

Email: info@benthamscience.net

Seyyed Shariatzadeh

Department of Parasitology, Mazandaran University of Medical Sciences

Email: info@benthamscience.net

Toktam Abbasi

Student in Medical Laboratory Sciences, School of Paramedics, Islamic Azad University Tehran Medical Sciences

Email: info@benthamscience.net

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