Prospects of Identifying Alternative Splicing Events from Single-Cell RNA Sequencing Data
- Autores: Wang J.1, Yuan L.2
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Afiliações:
- Department of Hepatobiliary Surgery, Quzhou People's Hospital
- Department of Hepatobiliary Surgery, Quzhou City People's Hospital
- Edição: Volume 19, Nº 9 (2024)
- Páginas: 845-850
- Seção: Life Sciences
- URL: https://vietnamjournal.ru/1574-8936/article/view/644073
- DOI: https://doi.org/10.2174/0115748936279561231214072041
- ID: 644073
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Texto integral
Resumo
Background:The advent of single-cell RNA sequencing (scRNA-seq) technology has offered unprecedented opportunities to unravel cellular heterogeneity and functions. Yet, despite its success in unraveling gene expression heterogeneity, accurately identifying and interpreting alternative splicing events from scRNA-seq data remains a formidable challenge. With advancing technology and algorithmic innovations, the prospect of accurately identifying alternative splicing events from scRNA-seq data is becoming increasingly promising.
Objective:This perspective aims to uncover the intricacies of splicing at the single-cell level and their potential implications for health and disease. It seeks to harness scRNA-seq's transformative power in revealing cell-specific alternative splicing dynamics and aims to propel our understanding of gene regulation within individual cells to new heights.
Methods:The perspective grounds its method on recent literature along with the experimental protocols of single-cell RNA-seq and methods to identify and quantify the alternative splicing events from scRNA-seq data.
Results:This perspective outlines the promising potential, challenges, and methodologies for leveraging different scRNA-seq technologies to identify and study alternative splicing events, with a focus on advancing our understanding of gene regulation at the single-cell level.
Conclusion:This perspective explores the prospects of utilizing scRNA-seq data to identify and study alternative splicing events, highlighting their potential, challenges, methodologies, biological insights, and future directions.
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Sobre autores
Jiacheng Wang
Department of Hepatobiliary Surgery, Quzhou People's Hospital
Autor responsável pela correspondência
Email: info@benthamscience.net
Lei Yuan
Department of Hepatobiliary Surgery, Quzhou City People's Hospital
Email: info@benthamscience.net
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