Therapeutic Effects of Arctiin on Alzheimer's Disease-like Model in Rats by Reducing Oxidative Stress, Inflammasomes and Fibrosis
- Авторы: Almeaqli M.1, Alaidaa Y.1, Alnajjar F.1, Al Shararh A.1, Alharbi D.1, Almslmani Y.1, Alotibi Y.1, Alrashidi H.1, Alshehri W.1, Hassan H.2, Al-Gayyar M.3
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Учреждения:
- PharmD Program, Faculty of Pharmacy, University of Tabuk
- Department of Pharmacology and Biochemistry, Faculty of Pharmacy, Delta University for Science and Technology
- Department of Biochemistry, Faculty of Pharmacy, Mansoura University
- Выпуск: Том 21, № 4 (2024)
- Страницы: 276-288
- Раздел: Medicine
- URL: https://vietnamjournal.ru/1567-2050/article/view/643790
- DOI: https://doi.org/10.2174/0115672050333388240801043509
- ID: 643790
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Аннотация
Background:Alzheimer's disease (AD) affects approximately 50 million people globally and is expected to triple by 2050. Arctiin is a lignan found in the Arctium lappa L. plant. Arctiin possesses anti-proliferative, antioxidative and anti-adipogenic.
Objectives:We aimed to explore the potential therapeutic effects of Arctiin on rats with AD by evaluating the expression of TLR4, NLRP3, STAT3, TGF-β, cyclin D1, and CDK2.
Methods:AD was induced in rats by administering 70 mg/kg of aluminum chloride through intraperitoneal injection daily for six weeks. After inducing AD, some rats were treated with 25 mg/kg of Arctiin daily for three weeks through oral gavage. Furthermore, to examine the brain tissue structure, hippocampal sections were stained with hematoxylin/eosin and anti-TLR4 antibodies. The collected samples were analyzed for gene expression and protein levels of TLR4, NLRP3, STAT3, TGF-β, cyclin D1, and CDK2.
Results:In behavioral tests, rats showed a significant improvement in their behavior when treated with Arctiin. Microimages stained with hematoxylin/eosin showed that Arctiin helped to improve the structure and cohesion of the hippocampus, which was previously impaired by AD. Furthermore, Arctiin reduced the expression of TLR4, NLRP3, STAT3, TGF-β, cyclin D1, and CDK2.
Conclusion:Arctiin can enhance rats behavior and structure of the hippocampus in AD rats. This is achieved through its ability to reduce the expression of both TLR4 and NLRP3, hence inhibiting the inflammasome pathway. Furthermore, Arctiin can improve tissue fibrosis by regulating STAT3 and TGF-β. Lastly, it can block the cell cycle proteins cyclin D1 and CDK2.
Об авторах
Mohamed Almeaqli
PharmD Program, Faculty of Pharmacy, University of Tabuk
Email: info@benthamscience.net
Yazeed Alaidaa
PharmD Program, Faculty of Pharmacy, University of Tabuk
Email: info@benthamscience.net
Faisal Alnajjar
PharmD Program, Faculty of Pharmacy, University of Tabuk
Email: info@benthamscience.net
Abdullah Al Shararh
PharmD Program, Faculty of Pharmacy, University of Tabuk
Email: info@benthamscience.net
Danah Alharbi
PharmD Program, Faculty of Pharmacy, University of Tabuk
Email: info@benthamscience.net
Yazeed Almslmani
PharmD Program, Faculty of Pharmacy, University of Tabuk
Email: info@benthamscience.net
Yousef Alotibi
PharmD Program, Faculty of Pharmacy, University of Tabuk
Email: info@benthamscience.net
Hani Alrashidi
PharmD Program, Faculty of Pharmacy, University of Tabuk
Email: info@benthamscience.net
Wael Alshehri
PharmD Program, Faculty of Pharmacy, University of Tabuk
Email: info@benthamscience.net
Hanan Hassan
Department of Pharmacology and Biochemistry, Faculty of Pharmacy, Delta University for Science and Technology
Email: info@benthamscience.net
Mohammed Al-Gayyar
Department of Biochemistry, Faculty of Pharmacy, Mansoura University
Автор, ответственный за переписку.
Email: info@benthamscience.net
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