5-methylthiopentyl Isothiocyanate, a Sulforaphane Analogue, Inhibits Pro-inflammatory Cytokines by Regulating LPS/ATP-mediated NLRP3 Inflammasome Activation
- Authors: Choi S.1, Kim J.1, Kwon S.2, Ahn N.3, Lee J.1, Yang W.4, Kim C.5, Yang S.2
-
Affiliations:
- Department of Biomedical Engineering, College of Life Science and Biotechnology,, Dongguk University
- Department of Biomedical Engineering, College of Life Science and Biotechnology, Dongguk University
- Department of Biomedical Engineering, College of Life Science and Biotechnology,, Dongguk University, Seoul,
- , National Institute for Nanomaterials Technology (NINT)
- , Department of Biological Sciences
- Issue: Vol 25, No 5 (2024)
- Pages: 645-654
- Section: Biotechnology
- URL: https://vietnamjournal.ru/1389-2010/article/view/644853
- DOI: https://doi.org/10.2174/1389201024666230824093927
- ID: 644853
Cite item
Full Text
Abstract
Background:Pro-inflammatory cytokines secreted from activated macrophages and astrocytes are crucial mediators of inflammation for host defense. Among them, the secretion of IL-1β, a major pro-inflammatory cytokine, is especially mediated by the activation of NLRP3 inflammasome. Pro-IL-1β, which is produced in response to the invaded pathogens, such as LPS, is cleaved and matured in the NLRP3 inflammasome by the recognition of ATP. Excessively activated IL-1β induces other immune cells, resulting in the up-regulation of inflammation. Therefore, regulation of NLRP3 inflammasome can be a good strategy for alleviating inflammation.
Objective:Our study aimed to examine whether 5-methylthiopentyl isothiocyanate, a sulforaphane analogue (berteroin), has an anti-inflammatory effect on the NLRP3 inflammasome activation induced by LPS and ATP.
Methods:Primary bone marrow-derived macrophages (BMDMs) and astrocytes were stimulated by LPS and ATP with the treatment of 5-methylthiopentyl isothiocyanate, a sulforaphane analogue. The secretion of pro-inflammatory cytokines was measured by ELISA, and the expression level of NLRP3 inflammasome-associated proteins was detected by western blot. The association of NLRP3 inflammasome was assessed by co-immunoprecipitation, and the formation of ASC specks was evaluated by fluorescent microscope.
Results:5-methylthiopentyl isothiocyanate, a sulforaphane analogue (berteroin), decreased the release of pro-inflammatory cytokines, IL-1β, and IL-6 in the BMDMs. Berteroin notably prevented the formation of both NLRP3 inflammasome and ASC specks, which reduced the secretion of IL-1β. Additionally, berteroin reduced the IL-1β secretion and cleaved IL-1β expression in the primary astrocytes.
Discussion and Conclusion:These results indicated the anti-inflammatory effects of 5- methylthiopentyl isothiocyanate (berteroin) by regulating NLRP3 inflammasome activation, suggesting that berteroin could be the potential natural drug candidate for the regulation of inflammation.
About the authors
Su-Bin Choi
Department of Biomedical Engineering, College of Life Science and Biotechnology,, Dongguk University
Email: info@benthamscience.net
Ji-Hye Kim
Department of Biomedical Engineering, College of Life Science and Biotechnology,, Dongguk University
Email: info@benthamscience.net
Sehee Kwon
Department of Biomedical Engineering, College of Life Science and Biotechnology, Dongguk University
Email: info@benthamscience.net
Na-Hyun Ahn
Department of Biomedical Engineering, College of Life Science and Biotechnology,, Dongguk University, Seoul,
Email: info@benthamscience.net
Joo-Hee Lee
Department of Biomedical Engineering, College of Life Science and Biotechnology,, Dongguk University
Email: info@benthamscience.net
Woong-Suk Yang
, National Institute for Nanomaterials Technology (NINT)
Email: info@benthamscience.net
Cheorl-Ho Kim
, Department of Biological Sciences
Author for correspondence.
Email: info@benthamscience.net
Seung-Hoon Yang
Department of Biomedical Engineering, College of Life Science and Biotechnology, Dongguk University
Author for correspondence.
Email: info@benthamscience.net
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