Ras-related Protein in Brain 4A (Rab4A) is Downregulated by miR-496 to inhibit the Progression of Gastric Cancer


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Abstract

Introduction:Ras-related protein in brain 4A (Rab4A), as a member of the Rab family, is involved in the intracellular circulation of membrane receptors or endocytic substances and regulates the progression of multiple tumors.

Methods:From our results, the knockdown of Rab4A inhibited the proliferation, migration and invasion in AGS cells. Importantly, the surface expression of epidermal growth factor receptor (EGFR) declined significantly in Rab4A knockdown cells. The downstream pathway of EGFR was also inhibited after the transfection of Rab4A-specific siRNA, including AKT and β-catenin pathways.

Result:In addition, miR-496 down-regulated the expression of Rab4A in AGS cells. The result of the luciferase reporter assay showed that miR-496 could bind to the 3’UTR of Rab4A.

Conclusion:In conclusion, the expression of Rab4A is inhibited by miR-496, and the knockdown of Rab4A inhibits the proliferation, migration and invasion through down-regulating the surface expression of EGFR. Rab4A is a potential target in the treatment of gastric cancer.

About the authors

Rui Su

Department of Gastrointestinal Surgery, Affiliated Hospital of Chengde Medical University

Email: info@benthamscience.net

Meng Su

Department of Pathogenic Biology, Chengde Medical University

Email: info@benthamscience.net

Yan Lu

Department of Anesthesiology, Affiliated Hospital of Chengde Medical College

Author for correspondence.
Email: info@benthamscience.net

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