Email this article 
Development of Quantitative Enzyme Immunoassay of Brain-Derived Neurotrophic Factor on the Basis of Recombinant Antigen
- Authors: Pavlova O.V.1, Murashko A.A.2, Andriushchenko N.V.1, Gurina O.I.1, Pavlov K.A.1
-
Affiliations:
- Serbsky National Medical Research Centre for Psychiatry and Narcology
- Moscow Research Institute of Psychiatry – the Branch of Serbsky National Medical Research Centre for Psychiatry and Narcology
- Issue: Vol 40, No 1 (2023)
- Pages: 86-91
- Section: МЕТОДЫ
- URL: https://vietnamjournal.ru/1027-8133/article/view/653960
- DOI: https://doi.org/10.31857/S1027813323010156
- EDN: https://elibrary.ru/EQWZUP
- ID: 653960
Cite item
Open Access
Access granted
Subscription Access
Abstract
One of the pathophysiological mechanisms of the development of some mental diseases, including depressive disorders, is a decrease in the expression of brain-derived neurotrophic factor (BDNF), mainly in the limbic region and prefrontal cortex of the brain. Immunochemical screening of BDNF in biological fluids showed that the levels of this protein can be considered as a marker of predisposition to depression and a prognostic marker of the effectiveness of therapy. The use of modern technologies for the production of recombinant proteins makes it possible to develop highly standardized ELISA systems for the determination of this antigen in biological fluids. This paper describes a method for creating a test system for quantitative ELISA of brain-derived neurotrophic factor on the basis of recombinant protein BDNF and antibodies obtained as a result of immunization with recombinant BDNF.
About the authors
O. V. Pavlova
Serbsky National Medical Research Centre for Psychiatry and Narcology
Email: nchjournal@gmail.com
Russia, Moscow
A. A. Murashko
Moscow Research Institute of Psychiatry – the Branch of SerbskyNational Medical Research Centre for Psychiatry and Narcology
Email: nchjournal@gmail.com
Russia, Moscow
N. V. Andriushchenko
Serbsky National Medical Research Centre for Psychiatry and Narcology
Email: nchjournal@gmail.com
Russia, Moscow
O. I. Gurina
Serbsky National Medical Research Centre for Psychiatry and Narcology
Email: nchjournal@gmail.com
Russia, Moscow
K. A. Pavlov
Serbsky National Medical Research Centre for Psychiatry and Narcology
Email: nchjournal@gmail.com
Russia, Moscow
References
- Kessler R.C., Bromet E.J. // Annu. Rev. Public. Health. 2013. V. 34. P. 113–138.https://doi.org/10.1146/annurev-publhealth-031912-114409
- Stetler C., Miller G.E. // Psychosom. Med. 2011. V. 73. № 2. P. 114–126. https://doi.org/10.1097/PSY.0b013e31820ad12b
- Irwin M.R., Miller A.H. // Brain Behav. Immun. 2007 V. 21. № 4. P. 374–383. https://doi.org/10.1016/j.bbi.2007.01.010
- Григорьян Г.А., Дыгало Н.Н., Гехт А.Б., Степаничев М.Ю., Гуляева Н.В. // Усп. физиол. наук. 2014. Т. 43. № 2. С. 3–19.
- Sullivan P.F., Agrawal A., Bulik C.M., Andreassen O.A., Borglum A.D., Breen G., Cichon S., Edenberg H.J., Faraone S.V., Gelernter J., Mathews C.A., Nievergelt C.M., Smoller J.W., O’Donovan M.C. // Am. J. Psychiatry. 2018. V. 175. № 1. P. 15–27. https://doi.org/10.1176/appi.ajp.2017.17030283
- Yang T., Nie Z., Shu H., Kuang Y., Chen X., Cheng J., Yu S., Liu H. // Front. Cell Neurosci. 2020. V. 14. P. 82. https://doi.org/10.3389/fncel.2020.00082.eCollection 2020
- Barde Y.A., Edgar D., Thoenen H. // EMBO J. 1982. V. 1. № 5. P. 549–53.
- Kowianski P., Lietzau G., Czuba E., Waskow M., Steliga A., Morys J. // Cell Mol. Neurobiol. 2018. V. 38. P. 579–593.https://doi.org/10.1007/s10571-017-0510-4
- Гуляева Н.В. // Биохимия. 2017. Т. 82. Bып. 3. С. 441–448.
- Taliaz D., Stall N., Dar D.E, Zangen A.// Mol. Psychiatry. 2010. V. 15. № 1. P. 80–92. https://doi.org/10.1038/mp.2009.67
- Filho C.B., Jesse C.R., Donato F., Giacomeli R., Del Fabbro L., da Silva Antunes M., de Gomes M.G., Goes A.T., Boeira S.P., Prigol M., Souza L.C. // Neuroscience. 2015. V. 289. P. 367–380. https://doi.org/10.1016/j.neuroscience.2014.12.048
- Yoshida T., Ishikawa M., Niitsu T., Nakazato M., Watanabe H., Shiraishi T., Shiina A., Hashimoto T., Kanahara N., Hasegawa T., Enohara M., Kimura A., Iyo M., Hashimoto K. // PLoS One. 2012. V. 7. № 8. e42676. https://doi.org/10.1371/journal.pone.0042676
- Duman R.S., Aghajanian G.K., Sanacora G., Krystal J.H. // Nat. Med. 2016. V. 23. № 3. P. 238–249. https://doi.org/10.1038/nm.4050
- Duman R.S., Deyama S., Fogaça M.V. // Eur. J. Neurosci. 2021. V. 53. № 1. P. 126–139. https://doi.org/10.1111/ejn.14630
- Mondal A.C., Fatima M. // Int. J. Neurosci. 2019. V. 129. № 3. P. 283–296. https://doi.org/10.1080/00207454.2018.1527328
- Castren E., Kojima M. // Neurobiol. Dis. 2017. V. 97. P. 119–126. https://doi.org/10.1016/j.nbd.2016.07.010
- Nobis A., Zalewski D., Waszkiewicz N. // J. Clin. Med. 2020. V. 9. 3793. https://doi.org/10.3390/jcm9123793
- Islam F., Mulsant B.H., Voineskos A.N., Rajji T.K. // Curr. Psychiatry Rep. 2017. V. 19. № 7. P. 36. https://doi.org/10.1007/s11920-017-0794-6
- Song J.H., Yu J.T., Tan L. // Mol. Neurobiol. 2015. V. 52. № 3. P. 1477–1493. https://doi.org/10.1007/s12035-014-8958-4
- Nuernberg G.L., Aguiar B., Bristot G., Fleck M.P., Rocha N.S. // Transl. Psychiatry. 2016. V. 6. № 12. e985. https://doi.org/10.1038/tp.2016.227
- Jin W. // J. Clin. Med. 2020. V. 9. № 1. P. 257. https://doi.org/10.3390/jcm9010257
Supplementary files
