Anxiogenic effect of pentylenetetrazole in a subconvulsive dose is accompanied by decreased cellular proliferation and neuronal NO-synthase expression in the posterior portion of the hippocampus
- Authors: Aniol V.А.1, Stepanichev М.Y.1, Yakovlev A.А.1, Lazareva N.А.1, Gulyaeva N.V.1
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Affiliations:
- Institute of Higher Nervous Activity and Neurophysiology, RAS
- Issue: Vol 41, No 3 (2024)
- Pages: 285-293
- Section: Experimental Articles
- URL: https://vietnamjournal.ru/1027-8133/article/view/653893
- DOI: https://doi.org/10.31857/S1027813324030081
- EDN: https://elibrary.ru/EQAXWN
- ID: 653893
Cite item
Abstract
In our previous studies, we have found a suppressive effect of a single administration of pentylenetetrazole (PTZ) in a subconvulsive dose on cellular proliferation in the dentate gyrus. In the present work, we show that this decrease in proliferation develops after acute anxiogenic effect of PTZ and is present only in the posterior portion of the hippocampus, where a decrease in the number of neuronal NO synthase expressing cells has been also found. These changes are also accompanied by a decrease in the level of nNOS protein in the hippocampus. Taken together, these observations may indicate the possible involvement of nNOS in the suppression of cellular proliferation in the dentate gyrus of the posterior hippocampus during the development of the anxiogenic effect of PTZ.
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About the authors
V. А. Aniol
Institute of Higher Nervous Activity and Neurophysiology, RAS
Author for correspondence.
Email: aniviktor@yandex.ru
Russian Federation, Moscow
М. Yu. Stepanichev
Institute of Higher Nervous Activity and Neurophysiology, RAS
Email: aniviktor@yandex.ru
Russian Federation, Moscow
A. А. Yakovlev
Institute of Higher Nervous Activity and Neurophysiology, RAS
Email: aniviktor@yandex.ru
Russian Federation, Moscow
N. А. Lazareva
Institute of Higher Nervous Activity and Neurophysiology, RAS
Email: aniviktor@yandex.ru
Russian Federation, Moscow
N. V. Gulyaeva
Institute of Higher Nervous Activity and Neurophysiology, RAS
Email: aniviktor@yandex.ru
Russian Federation, Moscow
References
- Eriksson P.S., Perfilieva E., Björk-Eriksson T., Alborn A.M., Nordborg C., Peterson D.A., Gage F.H. // Nat. Med. 1998. V. 4. № 11. P. 1313‒1317.
- Boldrini M., Fulmore C.A., Tartt A.N., Simeon L.R., Pavlova I., Poposka V, Rosoklija G.B., Stankov A., Arango V., Dwork A.J., Hen R., Mann J.J. // Cell Stem Cell. 2018. V. 22. № 4. P. 589−599.e5.
- Kempermann G., Gage F.H., Aigner L., Song H., Curtis M.A., Thuret S., Kuhn H.G., Jessberger S., Frankland P.W., Cameron H.A., Gould E., Hen R., Abrous D.N., Toni N., Schinder A.F., Zhao X., Lucassen P.J., Frisén J. // Cell Stem Cell. 2018. pii: S1934-5909(18)30166-8.
- Sorrells S.F., Paredes M.F., Cebrian-Silla A., Sandoval K., Qi D., Kelley K.W., James D., Mayer S., Chang J., Auguste K.I., Chang E.F., Gutierrez A.J., Kriegstein A.R., Mathern G.W., Oldham M.C., Huang E.J., Garcia-Verdugo J.M., Yang Z., Alvarez-Buylla A. // Nature. 2018. V. 555. № 7696. P. 377−381.
- Cameron H.A., Woolley C.S., McEwen B.S., Gould E. // Neuroscience. 1993. V. 56. № 2. P. 337–344.
- Gage F.H. // Science. 2000. V. 287. № 5457. P. 1433–1438.
- Scharfman H.E., McCloskey D.P. // Epilepsy Res. 2009. V. 85. № 2−3. P. 150−161.
- Yun S., Reynolds R.P., Masiulis I., Eisch A.J. // Nat. Med. 2016. V. 22. № 11. P. 1239–1247.
- Aniol V.A., Stepanichev M.Y., Lazareva N.A., Gulyaeva N.V. // Epilepsy Behav. 2011. V. 22. № 3. P. 433−441.
- Gould E., McEwen B.S., Tanapat P., Galea L.A., Fuchs E. // J. Neurosci. 1997. V. 17. № 7. P. 2492–2498.
- Brummelte S., Galea L.A. // Neuroscience. 2010. V. 168. № 3. P. 680–690.
- Aniol V.A., Stepanichev M.Y. // Neurochem. J. 2007. V. 1. P. 265–274.
- Gray W.P., Sundstrom L.E. // Brain Res. 1998. V. 790. P. 52−59.
- Paxinos G., Watson Ch. // The rat brain in stereotaxic coordinates. San Diego: Academic Press, Inc., 1998.
- Racine R.J. // Electroencephalogr. Clin. Neurophysiol. 1972. V. 32. P. 281−294.
- Ito M., Chiu T.H., Rosenberg H.C. // Neurochem. Res. 1986. V. 11. № 5. P. 637−646.
- Olsen R.W. // Mol. Cell. Biochem. 1981. V. 39. P. 261−279.
- Bazyan A.S., Zhulin V.V., Karpova M.N., Klishina N.Y., Glebov R.N. // Brain Res. 2001. V. 888. № 2. P. 212−220.
- Corda M., Orlandi M., Lecca D., Giorgi O. // J. Pharmacol. Exp. Ther. 1992. V. 262. P. 792−798.
- Ekonomou A., Smith A.L., Angelatou F. // Mol. Brain Res. 2001. V. 95. P. 27−35.
- Rodin E.A., Calhoun H.D. // J. Nerv. Ment. Dis. 1970. V. 150. № 6. P. 438−443.
- Rodin E.A., Rutledge L.T., Calhoun H.D. // Electroencephalogr. Clin. Neurophysiol. 1958. V. 10. № 4. P. 719−723.
- Benjamin D., Lal H., Meyerson L.R. // Life Sci. 1990. V. 47. № 3. P. 195−203.
- Giusti P., Guidetti G., Costa E., Guidotti A. // J. Pharmacol. Exp. Ther. 1991. V. 257. № 3. P. 1062−1068.
- Rodgers R.J., Cole J.C., Aboualfa K., Stephenson L.H. // Pharm. Biochem. Behav. 1995. V. 52. № 4. P. 805−813.
- Gould E., Gross C.G. // J. Neurosci. 2002. V. 22. № 3. P. 619−623.
- Snyder J.S., Radik R., Wojtowicz J.M., Cameron H.A. // Hippocampus. 2009. V. 19. № 4. P. 360−370.
- Snyder J.S., Ramchand P., Rabbett S., Radik R., Wojtowicz J.M., Cameron H.A. // Neurobiol. Aging. 2011. V. 32. № 6. P. 1149−1156.
- Jiang W., Xiao L., Wang J.C., Huang Y.G., Zhang X. // Neurosci. Lett. 2004. V. 367. P. 344−348.
- Bashkatova V., Vitskova G., Narkevich V., Vanin A., Mikoyan V., Rayevsky K. // J. Mol. Neurosci. 2000. V. 14. P. 183–190.
- Kaneko K., Itoh K., Berliner L.J., Miyasaka K., Fujii H. // Magn. Reson. Med. 2002. V. 48. P. 1051−1056.
- Яковлев А.А., Перегуд Д.И., Павлова Т.В., Гуляева Н.В. // Нейрохимия. 2004. Т. 21. № 1. С. 58−67.
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