Protective influence of the zinc complex of 1-butylvioluric acid on the development of the liver tissue culture in the presence of convulsive poisons

Capa

Citar

Texto integral

Resumo

Introduction. Currently, the actual problem is the search for substances that can be used as drugs for the pharmacological correction of the effects of cytostatic damage by convulsive poisons. It is known that the liver is the first organ to encounter any foreign molecule carried by the portal bloodstream, and it is the most damaged.

The aim of this work was to study the effect of the zinc complex of 1-butylvioluric acid on the development of an organotypic culture of rat liver tissue after poisoning with 2-(dimethylaminomethyl)phenyl ester of dimethylcarbamic acid hydrochloride.

Material and methods. White 3-month-old male Wistar rats were used in the experiment. To study the effects of the test substances, liver tissue was selected and the method of organotypic cultivation was applied. Poisoning with convulsive poisons was modeled using 2-(dimethylaminomethyl)phenyl ester of dimethylcarbamic acid hydrochloride and the zinc complex of 1-butylvioluric acid was used as a drug for pharmacological correction of the effects of liver tissue damage.

Results. The data obtained indicate that under the action of 2-(dimethylaminomethyl) phenyl ester of dimethylcarbamic acid hydrochloride, which simulates the effect of convulsive poisons on liver cells, their cell proliferation is inhibited. It was also found that the zinc complex of 1-butylvioluric acid eliminates the inhibitory effect of 2-(dimethylaminomethyl)phenyl ester of dimethylcarbamic acid hydrochloride on the proliferation of liver tissue culture.

Research limitations. The study was performed on a culture of rat liver cells; to extrapolate data to the whole organism, it is necessary to take into account the data of toxicodynamics and toxicokinetics.

Conclusion. Thus, the data obtained in the experiments prove the effectiveness of the use of the zinc complex of 1-butylvioluric acid as a drug for the pharmacological correction of the consequences of exposure to convulsive poisons, and also create a basis for its further study.

Compliance with ethical standards. Animals were kept in accordance with the requirements of GOST 33044–2014 dated 08/01/2015 “Principles of good laboratory practice”.

Author contribution. All co-authors made an equal contribution to the research and preparation of the article for publication.

Conflict of interests. The authors declare no conflict of interest. 

Funding. The study was not sponsored.

Received: September 1, 2022 / Accepted: January 2023 / Published: February 28, 2023

Sobre autores

Taisiia Kolbasova

Institute of Toxicology of Federal Medico-Biomedical Agency

Autor responsável pela correspondência
Email: tasia.pcma.le4@mail.ru
ORCID ID: 0000-0003-1646-1760

Candidate of Medical Sciences, Researcher, Laboratory of Biochemical Toxicology and Pharmacology of the «Institute of Toxicology» of Federal Medico-Biomedical Agency, 192019, St. Petersburg, Russian Federation.

e-mail: tasia.pcma.le4@mail.ru

Scopus Author ID: 57209853108

Rússia

Natalia Chalisova

Institute of Toxicology of Federal Medico-Biomedical Agency; Pavlov Institute of Physiology RAS

Email: ni_chalisova@mail.ru
ORCID ID: 0000-0002-2371-0043

Scopus Author ID: 7006310236

Rússia

Ekaterina Egozova

Herzen University

Email: ekaterina_egozova@mail.ru
ORCID ID: 0000-0002-0055-3778

Лаборант кафедры анатомии и физиологии человека Федерального государственного бюджетного образовательного учреждения высшего образования «Российский государственный педагогический университет им. А.И. Герцена», (Herzen University), 191186, г. Санкт-Петербург, Российская Федерация.

e-mail: ekaterina_egozova@mail.ru

Rússia

Polina Ivanova

Pavlov Institute of Physiology RAS

Email: ivanovapolina19@mail.ru
ORCID ID: 0000-0003-3308-440X

Scopus Author ID: 57203746785

Rússia

Konstantin Krasnov

Institute of Toxicology of Federal Medico-Biomedical Agency

Email: krasnov_tox@mail.ru
ORCID ID: 0000-0003-1503-2243

AuthorID: 355846

Rússia

Aleksandr Bespalov

Institute of Toxicology of Federal Medico-Biomedical Agency

Email: lab.mphb@toxicology.ru
ORCID ID: 0000-0003-3537-9930

AuthorID: 726761

Rússia

Bibliografia

  1. Petrov A.N., Voitsekhovich K.O., Melekhova A.S., Lissitzky D.S., Belskaya A.V., Mikhailova M.V. et al. Problems of diagnosing neurotoxic disorders – the consequences of poisoning with convulsive substances. Vestnik Rossijskoj Voenno-medicinskoj akademii. 2017; 3 (59): 211–7. (in Russian)
  2. Hernández A.F., Gil F., Lacasaña M., Rodríguez-Barranco M., Tsatsakis A.M., Requena M. et al. Pesticide exposure and genetic variation in xenobiotic-metabolizing enzymes interact to induce biochemical liver damage. Food and Chemical Toxicology. 2013; 61: 144–51. https://doi.org/10.1016/j.fct.2013.05.012
  3. Mohi-Ud-Din R., Mir R.H., Sawhney G., Dar M.A., Bhat Z.A. Possible Pathways of Hepatotoxicity Caused by Chemical Agents. Curr Drug Metab. 2019; 20(11): 867–79. https://doi.org/10.2174/1389200220666191105121653
  4. Kashuro V.A., Glushkov S.I., Kutsenko S.A., Karpishchenko A.I., Novikova T.M., Aksenov V.V. et al. The state of the glutathione system in the liver tissues of rats in acute cyclophosphamide poisoning. Toksikologicheskij vestnik. 2003; 4: 25–30. (in Russian)
  5. Adhyapok P., Fu X., Sluka J.P., Clendenon S.G., Sluka V.D., Wang Z. et al. A computational model of liver tissue damage and repair. PLoS One. 2020; 15(12): e0243451. https://doi.org/10.1371/journal.pone.0243451
  6. Chalisova N.I., Kozlov V.K., Mulik A.B., Zatsepin E.P., Kostrova T.A. Protective effect of encoded amino acids on the development of liver tissue culture in the presence of a cytostatic agent. Toksikologicheskij vestnik. 2020; 2(161): 48–53. https://doi.org/10.36946/0869-7922-2020-2-47-52 (in Russian)
  7. Chalisova N.I., Ryzhak G.A., Umnov R.S., Linkova N.S. Influence of tripeptides on the development of organotypic tissue culture of various origins. Molekulyarnaya medicina. 2022; 20(3): 30–3. https://doi.org/10.29296/24999490-2022-03-04 (in Russian)
  8. Bespalov A.Ya. Hydrochlorides of substituted 2-[(dimethylamino)methyl]aryldimethylcarbamates with anticholinesterase activity. Patent RF, No. 2754133; 2021. (in Russian)
  9. Krasnov K.A. et al. Carbon monoxide antidote. Patent RF, N 2581467; 2016.
  10. Kostrova T.A., Batotsyrenova E.G., Zolotoverkhaya E.A., Kubarskaya L.G., Kashuro V.A., Krasnov K.A. Effect of violuric acid on the antioxidant system in the long-term period after sodium thiopental poisoning. Medline.ru. Rossijskij biomedicinskij zhurnal. 2021; 22: 551–61. (in Russian)
  11. Kostrova T.A. Biochemical and behavioral indicators in the long-term period after acute poisoning by neurotoxicants and their pharmacological correction. Dr. med sci. diss. St. Petersburg, 2020. (in Russian)
  12. Kashuro V.A. Pathogenetic and diagnostic significance of the glutathione system in assessing the cytotoxic effect of anticancer drugs. Dr. med sci. diss. St. Petersburg; 2009. (in Russian)
  13. Kostrova T.A. Changes in the parameters of the antioxidant system and markers of neurotoxicity in the long-term period after severe poisoning with phenylcarbamate. In: Fundamental science and clinical medicine – Man and his health. Abstracts of the XXI International Biomedical Conference of Young Researchers [Fundamental`naya nauka i klinicheskaya medicina – Chelovek i ego zdorov`e. Tezisy` XXI Mezhdunarodnoj mediko-biologicheskoj konferencii molody`x issledovatelej]. 2018; 213–4. (in Russian)

Arquivos suplementares

Arquivos suplementares
Ação
1. JATS XML
Baixar

Declaração de direitos autorais © Kolbasova T.A., Chalisova N.I., Egozova E.S., Ivanova P.N., Krasnov K.A., Bespalov A.Y., 2023


СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).
Регистрационный номер и дата принятия решения о регистрации СМИ: серия ПИ № ФС 77 - 81728 от 11 декабря 2013.