Мақаланы E-mail арқылы жіберу DETERMINATION OF RICIN IN ENVIRONMENTAL SAMPLES USING BIOASSAY
- Авторлар: Rogovskaya N.Y.1, Gorbunov A.Y.1, Dubrovskii Y.A.1, Khlebnikova N.S.1, Babakov V.N.1
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Мекемелер:
- Research Institute of Hygiene, Occupational Pathology and Human Ecology, Federal Medical Biological Agency
- Шығарылым: № 5 (2020)
- Беттер: 43-49
- Бөлім: Articles
- ##submission.datePublished##: 05.11.2020
- URL: https://vietnamjournal.ru/0869-7922/article/view/641261
- DOI: https://doi.org/10.36946/0869-7922-2020-5-43-49
- ID: 641261
Дәйексөз келтіру
Толық мәтін
Аннотация
A bioassay of ricin toxicity in environmental samples using real-time cell index monitoring is proposed. The halfmaximal inhibitory concentration (IC50) of ricin was estimated at 6,7 ng/ml for human hepatoma HepaRG cells proliferation. The antibodies to A- and B-subunits in HepaRG cell media lead to cytoprotective and antiapoptotic effects against the cytotoxicity of ricin. The antibodies neutralised activation of JNK kinase (phosphorylated at Thr183/Tyr185) and prevented accumulation of the active forms of caspase 8 (hydrolysed to Asp384) and caspase 9 (hydrolysed to Asp315) induced by ricin in HepaRG cells. The tested antibodies also prevented a decrease in the intracellular levels of the active forms of Akt 1 kinase (phosphorylated at Ser473) and transcription factor p53 (phosphorylated at Ser46) caused by ricin. The bioassay with antibodies can be considered as a specific method for identifying the toxin in environmental samples.
Негізгі сөздер
Авторлар туралы
N. Rogovskaya
Research Institute of Hygiene, Occupational Pathology and Human Ecology, Federal Medical Biological Agency
Хат алмасуға жауапты Автор.
Email: niigpech@rihophe.ru
Rogovskaya Nadezhda Yur′evna
188663, Leningrad region
РесейA. Gorbunov
Research Institute of Hygiene, Occupational Pathology and Human Ecology, Federal Medical Biological Agency
Email: niigpech@rihophe.ru
Gorbunov Aleksandr Yur′evich
188663, Leningrad region
РесейYa. Dubrovskii
Research Institute of Hygiene, Occupational Pathology and Human Ecology, Federal Medical Biological Agency
Email: niigpech@rihophe.ru
Dubrovskii Yaroslav Aleksandrovich
188663, Leningrad region
РесейN. Khlebnikova
Research Institute of Hygiene, Occupational Pathology and Human Ecology, Federal Medical Biological Agency
Email: niigpech@rihophe.ru
Khlebnikova Natalia Semenovna
188663, Leningrad region
РесейV. Babakov
Research Institute of Hygiene, Occupational Pathology and Human Ecology, Federal Medical Biological Agency
Email: babakov@rihophe.ru
Babakov Vladimir Nikolaevich
188663, Leningrad region
РесейӘдебиет тізімі
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