DETERMINATION OF RICIN IN ENVIRONMENTAL SAMPLES USING BIOASSAY

Мұқаба

Дәйексөз келтіру

Толық мәтін

Аннотация

A bioassay of ricin toxicity in environmental samples using real-time cell index monitoring is proposed. The halfmaximal inhibitory concentration (IC50) of ricin was estimated at 6,7 ng/ml for human hepatoma HepaRG cells proliferation. The antibodies to A- and B-subunits in HepaRG cell media lead to cytoprotective and antiapoptotic effects against the cytotoxicity of ricin. The antibodies neutralised activation of JNK kinase (phosphorylated at Thr183/Tyr185) and prevented accumulation of the active forms of caspase 8 (hydrolysed to Asp384) and caspase 9 (hydrolysed to Asp315) induced by ricin in HepaRG cells. The tested antibodies also prevented a decrease in the intracellular levels of the active forms of Akt 1 kinase (phosphorylated at Ser473) and transcription factor p53 (phosphorylated at Ser46) caused by ricin. The bioassay with antibodies can be considered as a specific method for identifying the toxin in environmental samples.

Негізгі сөздер

Авторлар туралы

N. Rogovskaya

Research Institute of Hygiene, Occupational Pathology and Human Ecology, Federal Medical Biological Agency

Хат алмасуға жауапты Автор.
Email: niigpech@rihophe.ru

Rogovskaya Nadezhda Yurevna

188663, Leningrad region

Ресей

A. Gorbunov

Research Institute of Hygiene, Occupational Pathology and Human Ecology, Federal Medical Biological Agency

Email: niigpech@rihophe.ru

Gorbunov Aleksandr Yurevich

188663, Leningrad region

Ресей

Ya. Dubrovskii

Research Institute of Hygiene, Occupational Pathology and Human Ecology, Federal Medical Biological Agency

Email: niigpech@rihophe.ru

Dubrovskii Yaroslav Aleksandrovich

188663, Leningrad region

Ресей

N. Khlebnikova

Research Institute of Hygiene, Occupational Pathology and Human Ecology, Federal Medical Biological Agency

Email: niigpech@rihophe.ru

Khlebnikova Natalia Semenovna

188663, Leningrad region

Ресей

V. Babakov

Research Institute of Hygiene, Occupational Pathology and Human Ecology, Federal Medical Biological Agency

Email: babakov@rihophe.ru

Babakov Vladimir Nikolaevich

188663, Leningrad region

Ресей

Әдебиет тізімі

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© Rogovskaya N.Y., Gorbunov A.Y., Dubrovskii Y.A., Khlebnikova N.S., Babakov V.N., 2020



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