CHANGES IN REPRODUCTIVE POTENTIAL, SURVIVAL AND METABOLIC ACTIVITY IN TWO SUCCESSIVE GENERATIONS OF DAPHNIA MAGNA IN THE PIPERONYL BUTOXIDE MEDIUM

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Abstract

Changes in survival, fertility and metabolic activity in two generations of crustacean Daphnia magna in the piperonyl butoxide medium (PBO) were analyzed in concentrations of 50 to 800 ug/ml in a 21 day experiment in each generation. Metabolism disorders were evaluated using the MTT-assay traditionally applied in vitro to analyze cytotoxicity of medications. The MTT-assay shows in an integral way the amount of oxygen active forms, inactivation of mitochondrial oxidases, ratio of living and dead cells and function of antioxidant enzymes. It was found out that in the first generation, the PBO concentration of 353 ug/l is semi-lethal to Daphnia fertility and to the Daphnia survival it is 650 ug/l. In the second generation the toxic concentration is two-fold lower and amounts to 194 ug/l to fertility and to 200 ug/l to survival. It was revealed that PBO poses the cytotoxic effect on Daphnia.

About the authors

N. I. Kolesnikova

Obninsk Institute of Nuclear Power Engineering

Author for correspondence.
Email: Natasha442707245@mail.ru
249040 Obninsk, Kaluga region Russian Federation

A. O. Morozova

Obninsk Institute of Nuclear Power Engineering

Email: Sashkaa95@yandex.ru
249040 Obninsk, Kaluga region Russian Federation

D. V. Uskalova

Obninsk Institute of Nuclear Power Engineering

Email: uskalovad@mail.ru
249040 Obninsk, Kaluga region Russian Federation

E. I. Sarapultseva

Obninsk Institute of Nuclear Power Engineering;
National Research Nuclear University

Email: helen-bio@yandex.ru

249040 Obninsk, Kaluga region;

115409 Moscow

Russian Federation

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Copyright (c) 2017 Kolesnikova N.I., Morozova A.O., Uskalova D.V., Sarapultseva E.I.


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