Magnetic nanoparticles as a platform for delivery of the photosensitizer methylene blue to HCT116 tumor cells

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Resumo

Hybrid nanosystems based on magnetic iron oxide nanoparticles (IONPs) and human serum albumin (HSA), containing methylene blue (MB) as a model photosensitizer, have been synthesized. The resulting HSA@IONP nanosystems were characterized for size and composition using UV/visible spectrophotometry (particularly, using the Bradford method), dynamic light scattering, and electron magnetic resonance. A study of the dark and photoinduced cytotoxicity of MB, IONP, HSA@IONP, MB–IONP, MB–(HSA@IONP) on of human colon adenocarcinoma HCT116 cells was carried out. Under the experimental conditions, the difference between the dark and light-induced cytotoxicity of nanosystems on cells was significantly enhanced when the photosensitizer was immobilized on the surface of the carrier particles compared to free photosensitizer in equivalent concentrations.

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Sobre autores

M. Nguyen

Emanuel Institute of Biochemical Physics, Russian Academy of Sciences

Email: anna.v.bychkova@gmail.com
Rússia, Moscow

A. Markova

Emanuel Institute of Biochemical Physics, Russian Academy of Sciences

Email: anna.v.bychkova@gmail.com
Rússia, Moscow

B. Batchaeva

Emanuel Institute of Biochemical Physics, Russian Academy of Sciences

Email: anna.v.bychkova@gmail.com
Rússia, Moscow

M. Gorobets

Emanuel Institute of Biochemical Physics, Russian Academy of Sciences

Email: anna.v.bychkova@gmail.com
Rússia, Moscow

A. Toroptseva

Emanuel Institute of Biochemical Physics, Russian Academy of Sciences

Email: anna.v.bychkova@gmail.com
Rússia, Moscow

M. Motyakin

Emanuel Institute of Biochemical Physics, Russian Academy of Sciences

Email: anna.v.bychkova@gmail.com
Rússia, Moscow

M. Abdullina

Emanuel Institute of Biochemical Physics, Russian Academy of Sciences

Email: anna.v.bychkova@gmail.com
Rússia, Moscow

A. Bychkova

Emanuel Institute of Biochemical Physics, Russian Academy of Sciences

Autor responsável pela correspondência
Email: anna.v.bychkova@gmail.com
Rússia, Moscow

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2. Fig. 1. Survival of human colon adenocarcinoma HCT116 cells exposed to the studied samples in dark (a) and light (b) experiments. In the case of particles without MS (NCHO and HSA@NCHO), the cells were incubated with an amount of particles equivalent to particles with MS (MS–NCHO and MS–(HSA@NCHO), respectively). The names of the studied systems, their structures, and their designations present in the figures are given in the upper part of the figure.

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