A method for increasing the efficiency of selection of aptamers to cellular receptors
- Authors: Kuznetsova V.Е.1, Lebedev T.D.1, Shershov V.Е.1, Shtylev G.F.1, Shishkin I.Y.1, Miftahov R.A.1, Butvilovskaya V.I.1, Grechishnikova I.V.1, Zasedateleva О.А.1, Chudinov А.V.1
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Affiliations:
- Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
- Issue: Vol 51, No 3 (2025)
- Pages: 451-460
- Section: ОБЗОРНАЯ СТАТЬЯ
- URL: https://vietnamjournal.ru/0132-3423/article/view/686963
- DOI: https://doi.org/10.31857/S0132342325030081
- EDN: https://elibrary.ru/KQMRJH
- ID: 686963
Cite item
Abstract
A method has been proposed to increase the efficiency of selection of aptamers to cellular receptors by the cell-Selex method, in particular to the receptor tyrosine kinase c-KIT. The use of Tween 20 in buffer solutions in concentrations not exceeding 0.01%, as well as trypsinolysis of surface proteins at the stage of elution of the combinatorial library of oligonucleotides bound to the cell surface, led to an increase in the specificity of aptamers and a decrease in nonspecific sorption according to the results of fluorescence microscopy, thermofluorimetric analysis and high-precision sequencing.
Keywords
Full Text

About the authors
V. Е. Kuznetsova
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
Author for correspondence.
Email: kuzneimb@gmail.com
Russian Federation, ul. Vavilova 32, Moscow, 119991
T. D. Lebedev
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
Email: kuzneimb@gmail.com
Russian Federation, ul. Vavilova 32, Moscow, 119991
V. Е. Shershov
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
Email: kuzneimb@gmail.com
Russian Federation, ul. Vavilova 32, Moscow, 119991
G. F. Shtylev
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
Email: kuzneimb@gmail.com
Russian Federation, ul. Vavilova 32, Moscow, 119991
I. Yu. Shishkin
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
Email: kuzneimb@gmail.com
Russian Federation, ul. Vavilova 32, Moscow, 119991
R. A. Miftahov
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
Email: kuzneimb@gmail.com
Russian Federation, ul. Vavilova 32, Moscow, 119991
V. I. Butvilovskaya
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
Email: kuzneimb@gmail.com
Russian Federation, ul. Vavilova 32, Moscow, 119991
I. V. Grechishnikova
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
Email: kuzneimb@gmail.com
Russian Federation, ul. Vavilova 32, Moscow, 119991
О. А. Zasedateleva
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
Email: kuzneimb@gmail.com
Russian Federation, ul. Vavilova 32, Moscow, 119991
А. V. Chudinov
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
Email: kuzneimb@gmail.com
Russian Federation, ul. Vavilova 32, Moscow, 119991
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