DETERMINATION OF DOXORUBICIN BASED ON QUENCHING OF LUMINESCENCE OF ALLOYED QUANTUM DOTS

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Abstract

A sensitive, simple method for determining doxorubicin (Dox) in biological fluids, which does not require complex sample preparation, has been developed based on the quenching of luminescence of thioglycolic acid-stabilized CdZnSeS/ZnS quantum dots (QDs). Luminescence quenching was studied in model solutions and human blood plasma to establish optimal determination parameters. Optimal conditions for Dox detection were selected: QDs with optical density Aλ=360 = 0.05 and 25-fold plasma dilution. The developed method was applied to analyze a human blood sample. The limit of detection for Dox was 0.02 µg/mL, the limit of quantification 0.18 µg/mL, the linear range 0.27–4.07 µg/mL (R2 > 0.96); the method showed good reproducibility (sr from 1.08 to 1.19%). The method's accuracy was confirmed by HPLC with UV detection.

About the authors

D. G Koganova

Saratov State University named after N. G. Chernyshevsky

Email: Koganovadara43@gmail.com
Saratov, Russia

D. V Tsyupka

Saratov State University named after N. G. Chernyshevsky

Saratov, Russia

D. D Drozd

Saratov State University named after N. G. Chernyshevsky

Saratov, Russia

S. A Mescheriakova

Saratov State University named after N. G. Chernyshevsky

Saratov, Russia

P. S Pidenko

Saratov State University named after N. G. Chernyshevsky

Saratov, Russia

D. A Kornilov

Saratov State University named after N. G. Chernyshevsky

Saratov, Russia

O. A Goryacheva

Saratov State University named after N. G. Chernyshevsky

Saratov, Russia

I. Yu Goryacheva

Saratov State University named after N. G. Chernyshevsky

Saratov, Russia

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