Polymorphism of antioxidant defense enzyme genes as the risk for formation of chronic dust bronchitis in long-term labour experience coal mine workers
- Autores: Kazitskaya A.S.1,2, Yadykina T.K.1, Gulyaeva O.N.1, Panev N.I.1, Zhukova A.G.1,2
-
Afiliações:
- Research Institute for Complex Problems of Hygiene and Occupational Diseases
- Kuzbass Humanitarian and Pedagogical Institute of the FSBEI HE “Kemerovo State University”
- Edição: Volume 102, Nº 4 (2023)
- Páginas: 345-350
- Seção: OCCUPATIONAL HEALTH
- ##submission.datePublished##: 29.05.2023
- URL: https://vietnamjournal.ru/0016-9900/article/view/638579
- DOI: https://doi.org/10.47470/0016-9900-2023-102-4-345-350
- EDN: https://elibrary.ru/vdcjvh
- ID: 638579
Citar
Texto integral
Resumo
Introduction. The antioxidant defense system is involved in protecting the body from long-term exposure to harmful damaging factors. Polymorphisms in the genes of antioxidant defense enzymes change the response to oxidative stress in occupational respiratory diseases such as asbestosis, silicosis, occupational asthma, and others.
The aim was to study the polymorphism of the MnSOD (rs4880) and GPX1 (rs1050450) genes to predict the risk of developing chronic dust bronchitis in miners of the main occupations of coal enterprises in the South of Kuzbass.
Materials and methods. One hundred eighty two coal mine workers with long-term exposure to high concentrations of coal-rock dust (exceeding the maximum permissible concentrations by up to 35 times), including 116 people with a previously proven diagnosis of chronic dust bronchitis, were examined. The comparison group (66 workers) consisted of the persons without a proven diagnosis, working in the same sanitary and hygienic conditions. Genomic DNA was isolated from peripheral blood leukocytes using phenol-chloroform extraction method. Typing of the MnSOD (rs4880) and GPX1 (rs1050450) genes was performed using the Real-Time method.
Results. The chance of detecting the AA genotype of the MnSOD gene and the GG genotype of the GPX1 gene in long-term labour experience miners with chronic dust bronchitis was found to be 2 times and 6 times higher than in the comparison group, respectively. The AA GPX1 genotype had been shown to be a factor in resistance to the development of this disease. The combination of AA/GG genotypes of the MnSOD/GPX1 genes was statistically significantly associated with a 1.5-fold risk of developing chronic dust bronchitis. Combinations of genotypes of the MnSOD/GPX1 GG/AA, AA/AA, and AG/AA genes were associated with the resistance to the development of chronic dust bronchitis. The data obtained can be used for personalized prediction of the risk of developing chronic dust bronchitis in long-term labour experience miners of the main occupations.
Limitations. The study was limited to the number of miners who underwent periodic medical examinations and were hospitalized at the Research Institute for Complex Problems of Hygiene and Occupational Diseases.
Conclusion. The results obtained indicate to the contribution of the polymorphism of the MnSOD (rs4880) and GPX1 (rs1050450) genes to the development of chronic dust bronchitis.
Compliance with ethical standards. The examination of the patients complied with the ethical standards of the Bioethical Committee of the Research Institute for Complex Problems of Hygiene and Occupational Diseases, elaborated in accordance with the Helsinki Declaration of the World Association “Ethical Principles of Conducting Scientific Medical Research with Human Participation” as amended in 2013 and “Rules of Clinical Practice in the Russian Federation”, approved by the Order of the Ministry of Health of the Russian Federation No. 200n of April 01, 2016. Each study participant gave informed voluntary written consent to participate in the study and to publish personal medical information in depersonalized form in the Hygiene and Sanitation, Russian journal.
Contribution:
Kazitskaya A.S. — collection and processing of material, statistical processing, editing;
Yadykina T.K. — collection and processing of material;
Gulyaeva O.N. — collection and processing of material;
Panev N.I. — editing;
Zhukova A.G. — the concept and design of the study, writing a text.
All authors are responsible for the integrity of all parts of the manuscript and approval of the manuscript final version.
Conflict of interest. The authors declare no conflict of interest.
Acknowledgement. The study had no sponsorship.
Received: December 12, 2022 / Accepted: March 24, 2023 / Published: May 29, 2023
Sobre autores
Anastasiya Kazitskaya
Research Institute for Complex Problems of Hygiene and Occupational Diseases; Kuzbass Humanitarian and Pedagogical Institute of the FSBEI HE “Kemerovo State University”
Autor responsável pela correspondência
Email: noemail@neicon.ru
ORCID ID: 0000-0001-8292-4810
Rússia
Tatyana Yadykina
Research Institute for Complex Problems of Hygiene and Occupational Diseases
Email: noemail@neicon.ru
ORCID ID: 0000-0001-7008-1035
Rússia
Olga Gulyaeva
Research Institute for Complex Problems of Hygiene and Occupational Diseases
Email: noemail@neicon.ru
ORCID ID: 0000-0003-2225-6923
Rússia
Nikolaу Panev
Research Institute for Complex Problems of Hygiene and Occupational Diseases
Email: noemail@neicon.ru
ORCID ID: 0000-0001-5775-2615
Rússia
Anna Zhukova
Research Institute for Complex Problems of Hygiene and Occupational Diseases; Kuzbass Humanitarian and Pedagogical Institute of the FSBEI HE “Kemerovo State University”
Email: nyura_g@mail.ru
ORCID ID: 0000-0002-4797-7842
MD, PhD, DSci., Associate Professor, head of the molecular-genetic and experimental studies laboratory, Research Institute for Complex Problems of Hygiene and Occupational Diseases, Novokuznetsk, 654041, Russian Federation; head of the natural sciences sub-department, Kuzbass Humanitarian and Pedagogical Institute of the Kemerovo State University, Novokuznetsk, 654041, Russian Federation.
e-mail: nyura_g@mail.ru
RússiaBibliografia
- Kudaeva I.V., D’yakovich O.A., Katamanova E.V., Eshchina I.M. Impact of cardiovascular risk genes polymorphism in the development of metabolic disorders in persons exposed to vinyl chloride. Gigiena i Sanitaria (Hygiene and Sanitation, Russian journal). 2019; 98(10): 1113–8. https://doi.org/10.18821/0016-9900-2019-98-10-1113-1118 https://elibrary.ru/ijhyfw (in Russian)
- Kuz’mina L.P., Khotuleva A.G., Kovalevskiy E.V., Anokhin N.N., Tskhomariya I.M. Association of genetic polymorphism of cytokines and antioxidant enzymes with the development of asbestosis. Meditsina truda i promyshlennaya ekologiya. 2020; 60(12): 898–903. https://doi.org/10.31089/1026-9428-2020-60-12-898-903 https://elibrary.ru/ckpiuv (in Russian)
- Chernyak Yu.I. Association between HSPA1B, S100B, and TNF-α gene polymorphisms and risks of chronic mercury poisoning. Analiz riska zdorov’yu. 2021; (1): 126–32. https://doi.org/10.21668/health.risk/2021.1.13.eng https://elibrary.ru/icphjn
- Huang X. Iron, oxidative stress, and cell signaling in the pathogeneses of coal workers’ pneumoconiosis, silicosis, and asbestosis. Am. J. Biomed. Sci. 2011; 3(2): 95–106. https://doi.org/10.5099/aj110200095
- Kazitskaya A.S., Panev N.I., Yadykina T.K., Gulyaeva O.N., Evseeva N.A. Genetic and biochemical aspects of formation of professional chronic dust bronchitis. Meditsina truda i promyshlennaya ekologiya. 2019; 59(6): 342–47. https://doi.org/10.31089/1026-9428-2019-6-342-347 https://elibrary.ru/xeamhm (in Russian)
- Jin F.L., Zhang L.X., Chen K., Tian Y.G., Li J.S. Research progress in pathogenesis of pneumoconiosis. Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2020; 38(12): 948–52. https://doi.org/10.3760/cma.j.cn121094-20200219-00064 (in Chinese)
- Bondarev O.I., Bugaeva M.S., Kazitskaya A.S. The main pathogenetic mechanisms of pneumosclerotic changes in the lungs of coal industry workers. Meditsina truda i promyshlennaya ekologiya. 2022; 62(3): 177–84. https://doi.org/10.31089/1026-9428-2022-62-3-177-184 https://elibrary.ru/stzaxa (in Russian)
- Zhao H., Huang Y., Wang H., Zhao J., Tian S., Bai H., et al. Associations of SMAD4 rs10502913 and NLRP3 rs1539019 polymorphisms with risk of coal workers’ pneumoconiosis susceptibility in Chinese han population. Pharmgenomics Pers. Med. 2022; 15: 167–75. https://doi.org/10.2147/PGPM.S351658
- Yucesoy B., Luster M.I. Genetic susceptibility in pneumoconiosis. Toxicol. Lett. 2007; 168(3): 249–54. https://doi.org/10.1016/j.toxlet.2006.10.021
- Petsonk E.L., Rose C., Cohen R. Coal mine dust lung disease. New lessons from old exposure. Am. J. Respir. Crit. Care Med. 2013; 187(11): 1178–85. https://doi.org/10.1164/rccm.201301-0042CI
- Gafarov N.I., Zakharenkov V.V., Panev N.I., Kucher A.N., Freydin M.B., Rudko A.A. The role of genetic factors in the development of chronic dust bronchitis in workers of coal mining enterprises of Kuzbass. Gigiena i Sanitaria (Hygiene and Sanitation, Russian journal). 2013; 92(4): 44–7. https://elibrary.ru/rejswt (in Russian)
- Shpagina L.A. Actual problems in occupational lung diseases. Sibirskiy nauchnyy meditsinskiy zhurnal. 2017; 37(1): 55–60. https://elibrary.ru/xuwexr (in Russian)
- Sinitsky M.Y., Minina V.I., Asanov M.A., Yuzhalin A.E., Ponasenko A.V., Druzhinin V.G. Association of DNA repair gene polymorphisms with genotoxic stress in underground coal miners. Mutagenesis. 2017; 32(5): 501–9. https://doi.org/10.1093/mutage/gex018
- Kaur S., Gill M.S., Gupta K., Manchanda K. Effect of occupation on lipid peroxidation and antioxidant status in coal-fired thermal plant workers. Int. J. Appl. Basic. Med. Res. 2013; 3(2): 93–7. https://doi.org/10.4103/2229-516X.117065
- Saputri R.K., Setiawan B., Nugrahenny D., Kania N., Wahyuni E.S., Widodo M.A. The effects of Eucheuma cottonii on alveolar macrophages and malondialdehyde levels in bronchoalveolar lavage fluid in chronically particulate matter 10 coal dust-exposed rats. Iran J. Basic. Med. Sci. 2014; 17(7): 541–5.
- Zhukova A.G., Mikhailova N.N., Sazontova T.G., Zhdanova N.N., Kazitskaya A.S., Bugaeva M.S., et al. Participation of free-radical processes in structural and metabolic disturbances in the lung tissues caused by exposure to coal-rock dust and their adaptogenic correction. Bull. Exp. Biol. Med. 2020; 168(4): 439–43. https://doi.org/10.1007/s10517-020-04727-7
- Christiani D.C., Mehta A.J., Yu C.L. Genetic susceptibility to occupational exposures. Occup. Environ. Med. 2008; 65(6): 430–6; quiz 436, 397. https://doi.org/10.1136/oem.2007.033977
- Zhang H., Jin T., Zhang G., Chen L., Zou W., Li Q.Q. Polymorphisms in heat-shock protein 70 genes are associated with coal workers’ pneumoconiosis in southwestern China. In Vivo. 2011; 25(2): 251–7.
- Gaffney A., Christiani D.C. Gene-environment interaction from international cohorts: impact on development and evolution of occupational and environmental lung and airway disease. Semin. Respir. Crit. Care Med. 2015; 36(3): 347–57. https://doi.org/10.1055/s-0035-1549450
- Alhobeira H.A., Mandal R.K., Khan S., Dar S.A., Mahto H., Saeed M., et al. Link between MnSOD Ala16Val (rs4880) polymorphism and asthma risk is insignificant from sequential meta-analysis. Bioinformation. 2020; 16(11): 789–800. https://doi.org/10.6026/97320630016789
- Sambrook J., Russell D.W. Purification of nucleic acids by extraction with phenol:chloroform. CSH Protoc. 2006; 2006(1): pdb.prot4455. https://doi.org/10.1101/pdb.prot4455
- Zhukova A.G., Kazitskaya A.S., Yadykina T.K., Gulyaeva O.N., Panev N.I., Logunova T.D. Method for molecular genetic prediction of the risk of chronic dust bronchitis in coal mining workers. Patent RF № 2767916 C1; 2022. (in Russian)
- do Nascimento M.R., Silva de Souza R.O., Silva A.L. Jr., Lima E.S., Gonçalves M.S., de Moura Neto J.P. GSTP1 rs1695 and rs1871042, and SOD2 rs4880 as molecular markers of lipid peroxidation in blood storage. Blood Transfus. 2021; 19(4): 309–16. https://doi.org/10.2450/2020.0062-20
- de Oliveira M.A.A., Mallmann N.H., de Souza G.K.B.B., de Jesus Bacha T., Lima E.S., de Lima D.S.N., et al. Glutathione S-transferase, catalase, and mitochondrial superoxide dismutase gene polymorphisms modulate redox potential in systemic lupus erythematosus patients from Manaus, Amazonas, Brazil. Clin. Rheumatol. 2021; 40(9): 3639–49. https://doi.org/10.1007/s10067-021-05680-0
- Jablonska E., Gromadzinska J., Peplonska B., Fendler W., Reszka E., Krol M.B., et al. Lipid peroxidation and glutathione peroxidase activity relationship in breast cancer depends on functional polymorphism of GPX1. BMC Cancer. 2015; 15: 657. https://doi.org/10.1186/s12885-015-1680-4
- Bresciani G., Cruz I.B., de Paz J.A., Cuevas M.J., González-Gallego J. The MnSOD Ala16Val SNP: relevance to human diseases and interaction with environmental factors. Free Radic. Res. 2013; 47(10): 781–92. https://doi.org/10.3109/10715762.2013.836275
- Ekoue D.N., He C., Diamond A.M., Bonini M.G. Manganese superoxide dismutase and glutathione peroxidase-1 contribute to the rise and fall of mitochondrial reactive oxygen species which drive oncogenesis. Biochim. Biophys. Acta Bioenerg. 2017; 1858(8): 628–32. https://doi.org/10.1016/j.bbabio.2017.01.006
- Ravnik-Glavač M., Goričar K., Vogrinc D., Koritnik B., Lavrenčič J.G., Glavač D., et al. Genetic variability of inflammation and oxidative stress genes affects onset, progression of the disease and survival of patients with amyotrophic lateral sclerosis. Genes (Basel). 2022; 13(5): 757. https://doi.org/10.3390/genes13050757
Arquivos suplementares
