The β-actin and 36B4 Genes in the Soft Coral Sclerophytum heterospiculatum (Verseveldt, 1970)

Capa

Citar

Texto integral

Acesso aberto Acesso aberto
Acesso é fechado Acesso está concedido
Acesso é fechado Somente assinantes

Resumo

Coral polyps are the subject of various studies, including in the field of molecular biology. At the moment, much attention is being paid to molecular studies of corals of the Hexacorallia subclass. For this purpose, we identified and characterized the sequences of the β-actin and 36B4 genes from the soft coral Sclerophytum heterospiculatum (Verseveldt, 1970).The 36B4 and β-actin genes are necessary for the normal functioning of cells and are highly conservative between taxa, which are confirmed by the phylogenetic tree obtained in this work.

Palavras-chave

Texto integral

Acesso é fechado

Sobre autores

E. Bizikashvili

Zhirmunsky National Scientific Center of Marine Biology, Far East Branch, Russian Academy of Sciences

Autor responsável pela correspondência
Email: bilielena801@gmail.com
Rússia, Vladivostok, 690041

E. Shamshurina

Zhirmunsky National Scientific Center of Marine Biology, Far East Branch, Russian Academy of Sciences

Email: bilielena801@gmail.com
Rússia, Vladivostok, 690041

T. Sikorskaya

Zhirmunsky National Scientific Center of Marine Biology, Far East Branch, Russian Academy of Sciences

Email: bilielena801@gmail.com
Rússia, Vladivostok, 690041

Bibliografia

  1. Daly M., Brugler M.R., Cartwright P. et al. The phylum Cnidaria: A review of phylogenetic patterns and diversity 300 years after Linnaeus* // Zootaxa. 2007. V. 1668. P. 127–182. https://doi.org/10.5281/zenodo.180149
  2. Tursch B., Tursch A. The soft coral community on a sheltered reef quadrat at Laing Island (Papua New Guinea) // Mar. Biol. 1982. V. 68. P. 321–332. https://doi.org/10.1007/bf00409597
  3. Fabricius K.E. Soft coral abundance on the central Great Barrier Reef: Effects of Acanthaster planci, space availability, and aspects of the physical environment // Coral Reefs. 1997. V. 16. P. 159–167. https://doi.org/10.1007/s003380050070
  4. Boilard A., Dube C.E., Gruet C. et al. Defining coral bleaching as a microbial dysbiosis within the coral holobiont // Microorganisms. 2020. V. 8. https://doi.org/10.3390/microorganisms8111682
  5. Sikorskaya T.V., Ermolenko E.V. Changes of phospholipid molecular species profile upon bleaching and subsequent restoration of coral sinularia heterospiculata // Chem. Nat. Compd. 2024. V. 60. P. 215–219. https://doi.org/10.1007/s10600-024-04291-w
  6. Dean J.M., Lodhi I.J. Structural and functional roles of ether lipids // Protein Cell. 2018. V. 9. P. 196–206. https://doi.org/10.1007/s13238-017-0423-5
  7. Karge W.H., Schaefer E.J., Ordovas J.M. Quantification of mRNA by polymerase chain reaction (PCR) using an internal standard and a nonradioactive detection method // Methods Mol. Biol. 1998. V. 110. P. 43–61. https://doi.org/10.1385/1-59259-582-0:43
  8. Kozera B., Rapacz M. Reference genes in real-time PCR // J. Appl. Genet. 2013. V. 54. P. 391–406. https://doi.org/10.1007/s13353-013-0173-x
  9. Nguyen L.-T., Schmidt H.A., von Haeseler A. et al. IQ-TREE: A fast and effective stochastic algorithm for estimating maximum-likelihood phylogenies // Mol. Biol. Evol. 2015. V. 32. P. 268–274. https://doi.org/10.1093/molbev/msu300
  10. Hoang D.T., Chernomor O., von Haeseler A. et al. Ufboot2: Improving the ultrafast bootstrap appro-ximation // Mol. Biol. Evol. 2018. V. 35. P. 518–522. https://doi.org/10.1093/molbev/msx281
  11. Kalyaanamoorthy S., Minh B.Q., Wong T.K.F. et al. ModelFinder: Fast model selection for accurate phylogenetic estimates // Nat. Methods. 2017. V. 14. P. 587–589. https://doi.org/10.1038/nmeth.4285
  12. Gagou M., Ballesta J.P., Kouyanou S. Cloning and characterization of the ribosomal protein CcP0 of the medfly Ceratitis capitata // Insect. Mol. Biol. 2000. V. 9. P. 47–55. https://doi.org/10.1046/j.1365-2583.2000.00156.x
  13. Kabsch W., Vandekerckhove J. Structure and function of actin // Annu. Rev. Biophys. Biomol. Struct. 1992. V. 21. P. 49–76. https://doi.org/10.1146/annurev.bb.21.060192.000405
  14. Ishii K., Washio T., Uechi T. et al. Characteristics and clustering of human ribosomal protein genes // BMC Genomics. 2006. V. 7. P. 37. https://doi.org/10.1186/1471-2164-7-37

Arquivos suplementares

Arquivos suplementares
Ação
1. JATS XML
2. Fig. 1. Phylogenetic trees based on the maximum likelihood (ML) method for amino acid sequences of genes. a – 36B4, b – β-actin of various organisms; the numbers in the nodes indicate SH-aLRT support (%) / ultrafast bootstrap support (%).

Baixar (236KB)

Declaração de direitos autorais © Russian Academy of Sciences, 2025