Mechanisms of modulating action of thymoquinone (component of black cumin, Nigella sativa), affecting the activity of some nuclear and mitochondrial genes in mice tissue after exposure to X-Ray radiation

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The paper discusses a promising herbal preparation – thymoquinone, a component of black cumin (Nigella sativa), studied in experimental animals (mice, rats) in many pathologies, characterized by a positive effect and lack of toxic effect. The drug has been studied in a wide range of doses for injection and oral administration. Thymoquinone has antimicrobial, antiviral, anti-inflammatory, and radioprotective properties. The main damaging component of ionizing radiation is oxidative stress. For this reason, radioprotectors have recently been evaluated based on the drug’s ability to reduce oxidative stress. As markers of oxidative stress, we used parameters of changes in the expression of nuclear and mitochondrial DNA genes that perform essential functions in the cell. C57Bl/6 mice were administered thymoquinone (10 mg/kg) after 30 min. irradiation was performed (6 Gy). After 6 and 24 hours, gene expression in brain and spleen cells was studied using real-time PCR. It was shown that the activity of nuclear genes increased after exposure to radiation, but was normalized if thymoquinone was administered to mice 30 minutes before irradiation. Mitochondrial genes were also modified to target the activity of control cells. The test results show that thymoquinone has protective properties and may be promising as a radioprotector.

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Sobre autores

S. Abdullaev

Burnazyan Federal Medical Biophysical Center of Russia; Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences

Autor responsável pela correspondência
Email: saabdullaev@gmail.com
Rússia, Moscow; Moscow oblast, Pushchino

D. Fomina

Burnazyan Federal Medical Biophysical Center of Russia

Email: saabdullaev@gmail.com
Rússia, Moscow

N. Raeva

Burnazyan Federal Medical Biophysical Center of Russia

Email: saabdullaev@gmail.com
Rússia, Moscow

M. Popov

Vladimirsky Moscow Regional Research Clinical Institute

Email: saabdullaev@gmail.com
Rússia, Moscow

T. Maksimova

Sechenov First Moscow State Medical University

Email: saabdullaev@gmail.com
Rússia, Moscow

G. Zasukhina

Burnazyan Federal Medical Biophysical Center of Russia; Vavilov Institute of General Genetics, Russian Academy of Sciences

Email: saabdullaev@gmail.com
Rússia, Moscow; Moscow

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2. Fig. 1. Effect of TX on the expression of oncogenes (IAP-1, IkBa, NFKB (p50), iNOS) in the tissues of the spleen and brain of mice 6 and 24 hours after exposure to X-ray radiation at a dose of 6 Grams. The level of gene expression in unirradiated (control) mice was assumed to be 100%. The data is presented as an average ± SEM of 5-6 independent experiments. The differences from the control are statistically significant at p < 0.05 (*).

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3. 2. The effect of TC on the expression of mtDNA genes involved in oxidative phosphorylation (ND2, CYT-B, ATP6) in the tissues of the spleen and brain of mice, 6 and 24 hours after exposure to X-ray radiation at a dose of 6 Grams. The level of gene expression in unirradiated (control) mice was assumed to be 100%. The data is presented as an average ± SEM of 5-6 independent experiments. The differences from the control are statistically significant at p < 0.05 (*), p < 0.01 (**).

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