Influense of pre seeding electronic irradiation to the spring wheat sedlings and deseases insedence indicators

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. In the laboratory conditions of the climate chamber, a model experiment was conducted to study the effect of pre-sowing low-energy electron irradiation of seeds on the development indicators of spring wheat seedlings of the Iren variety. In the experiment, seeds naturally affected by root rot (pathogens Drechslera teres and Fusarium spp) were used. Irradiation in the range of 1–5 kGy was carried out on the electronic accelerator “Duet” at the ISE SB RAS, the radiation power is 100 100 Gy/pulse, at two electron energies – 100 keV (mode 1) and 120 keV (mode 2). The seeds were germinated in rolls of filter paper 9 and 12 days after irradiation. Unirradiated seeds served as a control. The repetition in the experiments is threefold. During the irradiation period of 9 days, there was a significant 1% increase in laboratory germination at doses of 2 and 4 kGy (electron energy 100 keV, mode 1) and at doses 1 and 4 kGy (electron energy 120 keV, mode 2), root lengths at doses 1 and 5 kGy (mode 1) by 4.3 and 3.4% and at doses of 1–3 kGy (mode 2) by 4–5% and there is no significant effect on the content of free proline and catalase activity in 7 daily wheat seedlings. During the irradiation period of 12 days, irradiation stimulated the length of the sprout at a dose of 2 kGy (mode 1) by 11.2%, and at doses of 5 kGy (mode 1) and 2–5 kGy (mode 2) it depressed by 12.2 and 20.4–32%, respectively. At doses of 3 and 5 kGy (mode 2), the length of the roots of seedlings decreased by 7.6 and 6.1%. Irradiation caused an increase in the crude mass of seedlings at doses of 1-5 kGy (mode 1) by 6.7–11.7% and at doses of 1 and 2 kGy (mode 2) by 8.7–17.8%, and at doses of 3–5 kGy (mode 2), on the contrary, a decrease of 21.6–32.3%. Taking into account the infestation of 7 daily wheat seedlings with diseases when laying at different times after irradiation of seeds showed that during the irradiation period of 12 days, the development of diseases was lower than during the period of 9 days.

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作者简介

Nadezhda Loy

Russian Institute of Radiology and Agroecology of National Research Centre «Kurchatov Institute»

编辑信件的主要联系方式.
Email: loy.nad@yandex.ru

PhD in Biological Sciences

俄罗斯联邦, Obninsk, Kaluga region

Natalia Sanzharova

Russian Institute of Radiology and Agroecology of National Research Centre «Kurchatov Institute»

Email: loy.nad@yandex.ru

Grand PhD in Biological Sciences, Corresponding Member of the RAS

俄罗斯联邦, Obninsk, Kaluga region

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2. Fig. 1. Effect of irradiation on sowing qualities of seeds with PP 9 days (a) and PP 12 days (b). * – differences are statistically significant compared to the control at P<0.5. The same in Figs. 2–6.

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3. Fig. 2. The effect of irradiation on the development of wheat seedlings at PP 9 days: a – mode 1 (100 keV), b – mode 2 (120 keV).

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4. Fig. 3. Effect of irradiation on the development of seedlings at PP 12 days.

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5. Fig. 4. Effect of irradiation on the content of free proline in wheat sprouts.

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6. Fig. 5. Infection of seedlings with helminthosporiosis (a) and fusarium (b), PP 9 days. P – infestation, P – prevalence (the same in Fig. 6).

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7. Fig. 6. Infection of seedlings with helminthosporiosis (a) and fusarium (b), PP 12 days.

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