STUDY OF THE LIPID PIGMENT COMPOSITION IN THE WHITE SEA ALGAE SACCHARINA LATISSIMA USING TLC AND MALDI-MS

Cover Page

Cite item

Full Text

Abstract

The qualitative composition of the most important biologically active lipid substances-carotenoids and chlorophyll derivatives of the White sea algaeSaccharina latissimahas been studied. The lipid extract was separated by thin-layer chromatography (TLC) and examined by matrix-associated laser desorption-ionization with mass-spectrometric analysis (MALDI-MS). The extract contained fucoxanthin, fucoxanthinol, pheophytin a, pheophorbide a, as well as other carotenoids and chlorophylls, including those not described in the literature. The results obtained, which significantly expand the information about the composition of S. latissima pigments, can be used to standardize raw materials and preparations based on these algae.

About the authors

К. А. Krasnov

Institute of Toxicology of the Federal Medical Biological Agency

Author for correspondence.
Email: krasnov_tox@mail.ru

Krasnov Konstantin Andreevich

192019, Saint Petersburg

Russian Federation

A. S. Gladchuk

Institute of Toxicology of the Federal Medical Biological Agency; St. Petersburg State University

Email: aleglad24@gmail.com

Gladchuk Aleksey Sergeevich

192019, Saint Petersburg

199034, Saint Petersburg

Russian Federation

M. L. Alexandrova

Institute of Toxicology of the Federal Medical Biological Agency

Email: analekt@mail.ru

Alexandrova Marina Leonidovna

192019, Saint Petersburg

Russian Federation

O. A. Keltsieva

Institute of Analytical Instrumentation, Russian Academy of Sciences; Institute of Toxicology of the Federal Medical Biological Agency

Email: keltcieva@gmail.com

Keltsieva Olga Alexandrovna

190103, Saint Petersburg

192019, Saint Petersburg

Russian Federation

M. A. Zaytseva

Institute of Toxicology of the Federal Medical Biological Agency

Email: alpha-2@mail.ru

Zaytseva Maria Anatolevna

192019, Saint Petersburg

Russian Federation

M. V. Melnikova

Institute of Toxicology of the Federal Medical Biological Agency

Email: margarita10108@mail.ru

Melnikova Margarita Viktorovna

192019, Saint Petersburg

Russian Federation

V. L. Reinyuk

Institute of Toxicology of the Federal Medical Biological Agency

Email: vladton@mail.ru

Reynyuk Vladimir Leonidovich

192019, Saint Petersburg

Russian Federation

Е. P. Podolskaya

Institute of Toxicology of the Federal Medical Biological Agency; Institute of Analytical Instrumentation, Russian Academy of Sciences

Email: ek.podolskaya@gmail.com

Podolskaya Ekaterina Petrovna

192019, Saint Petersburg

190103, Saint Petersburg

Russian Federation

References

  1. Bogolicin K.G., Kaplicyn P.A., Ul’yanovskij N.V., Pronina O.A. Complex research of the White sea brown algae chemical composition. Chemistry of plant raw materials. 2012; 4: 153-160 (in Russian).
  2. Bogolicin K.G., Kaplicyn P.A. Perspectives of using supercritical fluid extraction for obtaining of biologically active substances from the Arctic seas’ brown algae. In: Proceedings of the National school-conference of young scientists “Supercritical fluid technologies in the environmental management. Extraction of plant raw materials”. Arkhangelsk, June 25-28, 2012: 79-86 (in Russian).
  3. Stengel D.B., Connan S., Popper Z.A. Algal chemodiversity and bioactivity: sources of natural variability and implications for commercial application. Biotechnol Adv. 2011; 29(5): 483-501.
  4. Vadalà M., Palmieri B. From algae to “functional foods”. Clin Ter. 2015; 166(4): e281-300 (in Italian).
  5. Gammone M.A., Riccioni G., D’Orazio N. Marine Carotenoids against Oxidative Stress: Effects on Human Health. Mar. Drugs. 2015; 13(10): 6226-6246.
  6. Martin L.J. Fucoxanthin and Its Metabolite Fucoxanthinol in Cancer Prevention and Treatment. Mar. Drugs. 2015; 13(8): 4784-4798.
  7. Maeda H. Nutraceutical effects of fucoxanthin for obesity and diabetes therapy: a review. J. Oleo Sci. 2015; 64(2): 125-32.
  8. Abidov M., Ramazanov Z., Seifulla R., Grachev S. The effects of Xanthigen in the weight management of obese premenopausal women with non-alcoholic fatty liver disease and normal liver fat. Diabetes Obes Metab. 2010; 12(1): 72-81.
  9. Fujiwara T., Nishida N., Nota J., Kitani T., Aoishi K., Takahashi H., et.al. Efficacy of chlorophyll c2 for seasonal allergic rhinitis: single-center double-blind randomized control trial. Eur Arch Otorhinolaryngol. 2016; 273(12): 4289-4294.
  10. Islam M.N., Ishita I.J., Jin S.E., Choi R.J., Lee C.M., Kim Y.S., et.al. Antiinflammatory activity of edible brown alga Saccharina japonica and its constituents pheophorbide a and pheophytin a in LPSstimulated RAW 264.7 macrophage cells. Food Chem Toxicol. 2013; 55: 541-548.
  11. Lozovskaia М.Е. Effectiveness of using the biologically active additive to food from Laminaria in adolescents during complex treatment of the pulmonary tuberculosis. Nutrition issues. 2005; 74(1): 40-43 (in Russian).
  12. El-Nakeeb M.A., Jousef R.T. Antimicrobial activity of sodium cooper chlorophyllin. Pharmazie. 1974; 29: 48-50.
  13. Kamei Y., Aoki M.A. Сhlorophyll c2 analogue from the marine brown alga Eisenia bicyclis inactivates the infectious hematopoietic necrosis virus, a fish rhabdovirus. Arch. Virol. 2007; 152(5): 861-869.
  14. Murav’eva E.A. Complex technology for obtaining of extractive biologically active substances from the White Sea brown algae. Rybprom. 2010; 3: 54-57 (in Russian).
  15. Fu W., Magnúsdóttir M., Brynjólfson S., Palsson B.Ø., Paglia G. UPLC-UV-MS(E) analysis for quantification and identification of major carotenoid and chlorophyll species in algae. Anal Bioanal Chem. 2012; 404(10): 3145-3154.
  16. Milenković S.M., Zvezdanović J.B., Anđelković T.D., Marković D.Z. The identification of chlorophyll and its derivatives in the pigment mixtures: HPLC-chromatography, visible and mass spectroscopy studies. Advanced technologies. 2012; 1(1): 16-24.
  17. Murador D.C., Salafia F., Zoccali M., Martins P.L.G., Ferreira A.G., Dugo P., et.al. Green Extraction Approaches for Carotenoids and Esters: Characterization of Native Composition from Orange Peel. Antioxidants (Basel). 2019; 8(12): E613.

Supplementary files

Supplementary Files
Action
1. JATS XML
Download

Copyright (c) 2020 Krasnov К.А., Gladchuk A.S., Alexandrova M.L., Keltsieva O.A., Zaytseva M.A., Melnikova M.V., Reinyuk V.L., Podolskaya Е.P.


СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).
Регистрационный номер и дата принятия решения о регистрации СМИ: серия ПИ № ФС 77 - 81728 от 11 декабря 2013.