Rapid detection of A-type botulinum toxin using an aptasensor and SERS

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Resumo

We described the development of a biosensor for the rapid and sensitive detection of botulinum toxin type A. The sensor is a SERS substrate with an optimized concentration of labeled aptamers, immobilized on its surface. It allows the detection of botulinum toxin type A with a detection limit of 2.4 ng/ml in 1 hour.

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Sobre autores

O. Ambartsumyan

Moscow Institute of Physics and Technology

Autor responsável pela correspondência
Email: ambartsumian.oa@mipt.ru
Rússia, Moscow

A. Brovko

Moscow Institute of Physics and Technology

Email: ambartsumian.oa@mipt.ru
Rússia, Moscow

Bibliografia

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2. Fig. 1. Schematic diagram of the experiment

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3. Fig. 2. Ratio of SERS signal intensity of experiment and control

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4. Fig. 3. Distribution of SERS signal intensity from the aptamer solution to BoNT with concentration of 35.0 nM on the substrate

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5. Fig. 4. SERS signal intensity distribution from the aptamer solution to BoNT with 17.5 nM concentration on the substrate

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6. Fig. 5. SERS signal intensity distribution from the aptamer solution to BoNT with 8.8 nM concentration on the substrate

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7. Fig. 6. SERS signal intensity distribution of experiment and control for BoNT A (2.4 ng/ml) detection using a solution of aptamers with a concentration of 17.5 nM on the substrate

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8. Fig. 7. Distribution of SERS intensity of experiment and control in the determination of IgGHum (24 ng/ml) using a solution of aptamers to BoNT with a concentration of 17.5 nM on the substrate

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9. Fig. 8. Distribution of SERS intensity of the experiment and control in the determination of BoNT A (2.4 ng/ml) using a solution of aptamers to RSV with a concentration of 17.5 nM

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