Organization of the Reserve Pool of Synaptic Vesicles in Nerve Terminals Lacking Protein Liquid Phase Components

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Protein endophilin A regulates the synaptic vesicle cycle during exo- and endocytosis, and it is present in the reserve pool of synaptic vesicles (SVs), where its function is unknown. In vitro experiments suggest that endophilin via its SH3 domain interactions incorporates several components into the protein liquid phase that organizes SVs in the reserve pool. We investigated the effect of deletion of the genes encoding endophilin and one of its binding partners, dynamin, on the organization of SVs in living synapses formed by cortical neurons in culture. Experiments showed that deletion of endophilin genes does not change the density of SVs in the reserve pool. At the same time, deletion of the major dynamins 1 and 3 leads to a significant increase in the vesicle density. These results suggest that other SH3-domain-containing proteins, which are components of the protein liquid phase, complement the function of endophilin in the SV reserve pool.

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作者简介

N. Nifantova

Institute of Translational Biomedicine, St. Petersburg State University

编辑信件的主要联系方式.
Email: oleg.shupliakov@ki.se
俄罗斯联邦, St. Petersburg, 199034

A. Shishkov

Institute of Translational Biomedicine, St. Petersburg State University

Email: oleg.shupliakov@ki.se
俄罗斯联邦, St. Petersburg, 199034

O. Korenkova

Institute of Translational Biomedicine, St. Petersburg State University

Email: oleg.shupliakov@ki.se
俄罗斯联邦, St. Petersburg, 199034

E. Sopova

Institute of Translational Biomedicine, St. Petersburg State University; Karolinska Institutet

Email: oleg.shupliakov@ki.se
俄罗斯联邦, St. Petersburg, 199034; Stockholm, 17177, Sweden

L. Brodin

Karolinska Institutet

Email: oleg.shupliakov@ki.se
瑞典, Stockholm, 17177

O. Shupliakov

Institute of Translational Biomedicine, St. Petersburg State University; Karolinska Institutet

Email: oleg.shupliakov@ki.se
俄罗斯联邦, St. Petersburg, 199034; Stockholm, 17177, Sweden

参考

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2. Fig. 1. Effects of deletion of endophilin and dynamin genes on the organization of the CB reserve pool in asymmetric synapses in cortical neuron culture.

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