Mass spectrometric analysis of Xenopus laevis cytoskeletal protein zyxin post-translational modifications
- 作者: Ivanova E.D.1, Zyganshin R.H.2, Parshina E.A.2, Zaraisky A.G.2, Martynova N.Y.2
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隶属关系:
- Pirogov Russian National Research Medical University
- Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences
- 期: 卷 51, 编号 3 (2025)
- 页面: 388-397
- 栏目: ОБЗОРНАЯ СТАТЬЯ
- URL: https://vietnamjournal.ru/0132-3423/article/view/686890
- DOI: https://doi.org/10.31857/S0132342325030027
- EDN: https://elibrary.ru/KPZTMP
- ID: 686890
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详细
In addition to its involvement in fundamental cellular processes, zyxin, a LIM-domain protein in the cytoskeletal system, is actively studied because it plays an important role in mechanosensory functions, actin polymerization regulation at cell junctions, as well as gene expression regulation. The disruption of zyxin expression and processing has been associated with carcinogenesis and cardiovascular disease. Zyxin plays an important role in the invasion and metastasis of tumors. The post-translational modification of zyxin in mammals regulates its activity and subcellular location. Given that zyxin is an evolutionarily highly conserved protein, we conducted a search for post-translational modifications of the zyxin homolog from Xenopus laevis using chromatographic mass spectrometry. To identify modified peptides, an enrichment method was employed using co-immunoprecipitation of endogenous zyxin from gastrula-stage embryonic cell lysates. As a result, previously unknown modifications of this protein were discovered, specifically N-terminal acetylation at methionine position 1 and phosphorylation at Ser197 and Ser386. To identify zyxin isoforms with different electrophoretic mobilities, separation was performed using polyacrylamide gel electrophoresis. Zyxin was found in bands with electrophoretic mobilities of 70 and 105 kDa. Thus, this study presents entirely new data on the post-translational modifications of zyxin from X. laevis. Since defects in mechanical signal transduction are associated with developmental disorders, oncogenesis, and metastasis, the study of mechanosensitive protein zyxin modifications and processing on the model organism X. laevis opens up opportunities for diagnostic studies at the molecular level, which can be used in the future to determine drugs use prospective in pharmacology.
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作者简介
E. Ivanova
Pirogov Russian National Research Medical University
Email: martnat61@gmail.com
俄罗斯联邦, ul. Ostrovitianova 1, Moscow, 117997
R. Zyganshin
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences
Email: martnat61@gmail.com
俄罗斯联邦, ul. Miklukho-Maklaya 16/10, Moscow, 117997
E. Parshina
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences
Email: martnat61@gmail.com
俄罗斯联邦, ul. Miklukho-Maklaya 16/10, Moscow, 117997
A. Zaraisky
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences
Email: martnat61@gmail.com
俄罗斯联邦, ul. Miklukho-Maklaya 16/10, Moscow, 117997
N. Martynova
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences
编辑信件的主要联系方式.
Email: martnat61@gmail.com
俄罗斯联邦, ul. Miklukho-Maklaya 16/10, Moscow, 117997
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