Cell and Tissue BiologyCell and Tissue BiologyЦитология0041-37713034-6061The Russian Academy of Sciences68217110.31857/S0041377125010031DEYARNArticlesСтатьиResearch ArticleRedox status and accumulation of autophagosomes in the liver of mouse under the action of lithium chlorideРедокс-состояние и накопление аутофагосом в печени мыши при действии хлорида литияDmitrievaS. А.ДмитриеваС. А.
Russian Federation

Kazan Institute of Biochemistry and Biophysics

Казанский институт биохимии и биофизики

s_dmitrieva@list.ruPonomarevaA. A.ПономареваА. А.
Russian Federation

Kazan Institute of Biochemistry and Biophysics

Казанский институт биохимии и биофизики

s_dmitrieva@list.ruFRC Kazan Scientific Center, Russian Academy of SciencesФедеральный исследовательский центр «Казанский научный центр Российской академии наук»23032025671304103062025Copyright ©; 2025, Russian Academy of SciencesCopyright ©; 2025, Российская академия наук2025Russian Academy of SciencesРоссийская академия наукhttps://vietnamjournal.ru/0041-3771/article/view/682171

Activation of autophagy is considered one of the promising strategies for the treatment and prevention of various non-infectious liver diseases. In this work, we assessed the changes in redox status and autophagy activation in liver tissues in vivo under the action of lithium chloride. It was shown that lithium chloride leads to the accumulation of autophagosomes in liver cells under normal conditions. This process is accompanied by a slight increase in the activity of several antioxidant enzymes. Toxic effects on the liver and the development of oxidative stress with 3-day use of LiCl were not detected. Significant rearrangements in the ultrastructure of the endoplasmic reticulum were observed, which can play a signaling role and participate in the initiation of autophagy. Thus, oral application of lithium chloride can be used as an effective modulator of the autophagy process in liver tissues.

Активация аутофагии считается одной из перспективных стратегией лечения и профилактики разнообразных неинфекционных заболеваний печени. В работе проведена оценка изменения редокс-состояния и активации аутофагии в тканях печени в условиях in vivo при действии хлористого лития (LiCl) в течение трех суток. Использовали просвечивающую электронную микроскопию для оценки образования аутофагических вакуолей в гепатоцитах печени мыши и ряд биохимических методов для определения в крови или печени активности ферментов, содержания глюкозы, пероксида водорода, уровня окислительной модификации белков и перекисного окисления липидов. Показано, что пероральное применение различных концентраций LiCl приводит к накоплению аутофагосом в клетках печени и небольшому усилению активности ряда антиоксидантных ферментов. Наблюдали значимые перестройки в ультраструктуре эндоплазматического ретикулума, которые могут играть сигнальную роль и участвовать в запуске аутофагии. Токсического действия на печень и развития окислительного стресса при применении LiCl в течение трех суток не обнаружено. Пероральное применение LiCl может использоваться в качестве эффективного модулятора процесса аутофагии в тканях печени.

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