Cell and Tissue BiologyCell and Tissue BiologyЦитология0041-37713034-6061The Russian Academy of Sciences67747210.31857/S0041377124050091DUEIYYArticlesСтатьиResearch ArticleFeatures of the distribution of GABA and the α1 subunit of the GABAA receptor in the CA1 and CA3 fields of the hippocampus in newborn rats after asphixia in the neonatal periodОсобенности распределения ГАМК и α1-субъединицы ГАМКА-рецептора в полях СА1 и СА3 гиппокампа у новорожденных крыс после асфиксии в неонатальный периодKhozhayL. I.ХожайЛ. И.
Russian Federation
astarta0505@mail.ruPavlov Institute of Physiology of the Russian Academy of SciencesИнститут физиологии им. И.П. Павлова РАН15112024665-649150220032025Copyright ©; 2024, Russian Academy of SciencesCopyright ©; 2024, Российская академия наук2024Russian Academy of SciencesРоссийская академия наукhttps://vietnamjournal.ru/0041-3771/article/view/677472

A study was conducted of the dynamics of changes in the population of GABAergic neurons and the protein content of the α1 subunit, which is included in of the GABAA receptor (GABAAα1) in the CA1 and CA3 fields of the hippocampus during the neonatal period under normal conditions and after exposure to perinatal hypoxia. The study used a model of human premature pregnancy. Exposure to hypoxia was carried out on the 2nd day after birth, in a special chamber with oxygen content in the respiratory mixture of 7.8%. Immunohistochemical research methods were used to detect GABA and the α1 GABAA receptor subunit protein. The hippocampus was studied on days 5 and 10. It was shown that in control animals during the neonatal period, in fields CA1 and CA3, there is a gradual increase in the population of GABAergic neurons, an increase in the content of GABA itself and the protein of the α1 GABAA receptor subunit. Asphyxia during the perinatal period leads to a reduction in the number of GABAergic neurons in both fields CA1 and CA3, a decrease in the content of GABA itself, the protein of the α1 subunit of the GABAA receptor and a delay in the development of the neuropil. Thus, in animals that have experienced asphyxia, by the end of the neonatal period, changes in the organization of the GABAergic system are already expressed in parts of the hippocampus, which can lead to dysfunction of the inhibitory system already at the earliest stages of development.

Проведено исследование динамики изменения численности популяции ГАМКергических нейронов и распределения субъединицы α1, входящей в состав ГАМКA-рецептора (ГАМКAα1) в полях СА1 и СА3 гиппокампа во время неонатального периода в норме и после воздействия перинатальной гипоксии. В работе использована модель недоношенной беременности человека. Воздействие гипоксии осуществляли на 2-е сутки после рождения, в специальной камере с содержанием кислорода в дыхательной смеси 7.8%. Для выявления ГАМК и субъединицы α1 ГАМКA-рецептора применяли иммуногистохимические методы исследования. Изучение гиппокампа проводили на 5 и 10 сутки. Показано, что у животных в контроле в течение неонатального периода в полях СА1 и СА3 происходит постепенное увеличение численности популяции ГАМКергических нейронов, повышение содержания самой ГАМК и субъединицы α1 ГАМКА рецептора. Асфиксия в перинатальный период приводит к сокращению числа ГАМКергических нейронов в обоих полях СА1 и СА3, снижению содержания самой ГАМК, субъединицы α1 ГАМКА-рецептора и задержке развития нейропиля. Таким образом у животных, переживших асфиксию, к концу неонатального периода в исследованных отделах гиппокампа уже выражено изменение организации ГАМКергической системы, которое может приводить к дисфункции тормозной системы уже на самых ранних этапах развития.

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