Cell and Tissue BiologyCell and Tissue BiologyЦитология0041-37713034-6061The Russian Academy of Sciences67746710.31857/S0041377124050044DUTHUGArticlesСтатьиResearch ArticleMicrovesicles from mesenchymal stem cells for cartilage tissue regeneration in equine osteoarthritisМикровезикулы из мезенхимных стволовых клеток для регенерации хрящевой ткани при остеоартрите лошадейAimaletdinovA. M.АймалетдиновА. М.
Russian Federation
aimaletdinowam@gmail.comMalanyevaA. G.МаланьеваА. Г.
Russian Federation
aimaletdinowam@gmail.comTambovskyM. A.ТамбовскийМ. А.
Russian Federation
aimaletdinowam@gmail.comZakirovaE. Yu.ЗакироваЕ. Ю.
Russian Federation
aimaletdinowam@gmail.comKazan (Volga Region) Federal UniversityКазанский (Приволжский) федеральный университет15112024665-643844920032025Copyright ©; 2024, Russian Academy of SciencesCopyright ©; 2024, Российская академия наук2024Russian Academy of SciencesРоссийская академия наукhttps://vietnamjournal.ru/0041-3771/article/view/677467

Current treatment strategies for osteoarthritis primarily focus on symptom management. Currently, the use of cell therapy methods, including mesenchymal stem cells (MSCs), is practiced in medicine and veterinary medicine. Microvesicles (MVs) obtained from MSCs are also currently used for the purpose of regeneration. The purpose of this study was to investigate the potential effects of artificial MVs on rat chondrocytes. In vitro experiments showed that MVs obtained from MSCs had a positive effect on the viability and migration ability of the chondrocyte cell culture. In 3D modeling of OA in vitro, MVs neutralized the effect of pro-inflammatory factors IL-1b and TNF-α. Most likely, these effects were due to the direct penetration of MVs contents into chondrocytes, since the possibility of fusion of MVs membranes with chondrocyte membranes was experimentally demonstrated. Thus, we have shown the positive effect of MVs on an in vitro model of OA.

Современные стратегии лечения остеоартрита (ОА) в основном направлены на устранение симптомов. В настоящее время в медицине и ветеринарии практикуется использование клеточных методов терапии, включающих мезенхимные стволовые клетки (МСК). Также в настоящее время с целью регенерации применяют микровезикулы (МВ), полученные из МСК. Цель настоящего исследования – изучить возможность потенциального влияния искусственных МВ на хондроциты крысы. Проведенные in vitro эксперименты показали, что МВ, полученные из МСК, оказывали положительное действие на жизнеспособность и миграционную способность культуры клеток хондроцитов. МВ in vitro при 3D-моделировании ОА нивелировали действие провоспалительных факторов IL-1b и TNF-α. Эксперименты показали, что МВ взаимодействуют и сливаются непосредственно с мембранами хондроцитов. Таким образом, нами показано влияние МВ из МСК лошади на хондроциты in vitro.

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