Cell and Tissue BiologyCell and Tissue BiologyЦитология0041-37713034-6061The Russian Academy of Sciences66960910.31857/S0041377124020019RKMSZJArticlesСтатьиResearch ArticleUmbilical blood as a trophic-growth supplement for cultural workПуповинная кровь как трофико-ростовая добавка для проведения культуральных работGoncharovA. G.ГончаровА. Г.
Russian Federation

Center of Immunology and Cell Biotechnology

Центр иммунологии и клеточных биотехнологий

larisalitvinova@yandex.ruShupletsovaV. V.ШуплецоваВ. В.
Russian Federation

Center of Immunology and Cell Biotechnology

Центр иммунологии и клеточных биотехнологий

larisalitvinova@yandex.ruGazatovaN. D.ГазатоваН. Д.
Russian Federation

Center of Immunology and Cell Biotechnology

Центр иммунологии и клеточных биотехнологий

larisalitvinova@yandex.ruMelashchenkoO. B.МелащенкоО. Б.
Russian Federation

Center of Immunology and Cell Biotechnology

Центр иммунологии и клеточных биотехнологий

larisalitvinova@yandex.ruYurovaK. A.ЮроваК. А.
Russian Federation

Center of Immunology and Cell Biotechnology

Центр иммунологии и клеточных биотехнологий

larisalitvinova@yandex.ruLitvinovaL. S.ЛитвиноваЛ. С.
Russian Federation

Center of Immunology and Cell Biotechnology; Laboratory of Cellular and Microfluidic Technologies

Центр иммунологии и клеточных биотехнологий; Лаборатория клеточных и микрофлюидных технологий

larisalitvinova@yandex.ruImmanuel Kant Baltic Federal UniversityБалтийский федеральный университет им. И. КантаSiberian State Medical UniversityСибирский государственный медицинский университет1503202466210712127022025Copyright ©; 2024, Russian Academy of SciencesCopyright ©; 2024, Российская академия наук2024Russian Academy of SciencesРоссийская академия наукhttps://vietnamjournal.ru/0041-3771/article/view/669609

This review analyzes the results of modern high-tech research on the use of umbilical cord blood serum/plasma as an additive to culture media for cell culture growth. Since culture media are a key factor in cell culture, the review addresses the composition and properties of the major culture media used in cell biology and regenerative medicine. The authors pay special attention to growth factors; they describe the functional properties of the main families of these polypeptides (fibroblast growth factors, epidermal growth factors, transforming growth factors, differentiation growth factors, epidermal growth factors, endothelial cell growth factors, hematopoietic growth factors, etc.). It was found that one of the most promising sources of growth factors is cord blood serum/plasma. In this publication, the main technologies for cord blood collection and systematic studies on the content of growth factors, cytokines, exosomes and mRNA in cord blood are presented. Experimental data on the use of umbilical cord blood serum/plasma as an additive to culture media for the growth of various cell cultures of animal origin are described. Human umbilical cord blood serum/plasma is an affordable, safe product with a high content of biologically active molecules compared to animal sources. In order for umbilical cord blood serum/plasma to be widely used as an adjunct to culture media, standards for the manufacture and testing of this product must be developed.

В обзоре проанализированы результаты современных высокотехнологичных исследований по применению сыворотки (плазмы) пуповинной крови в качестве добавки к культуральным средам для выращивания клеточных культур. Поскольку питательные среды являются ключевым фактором культивирования клеток, в обзоре рассматривается состав и свойства основных культуральных сред, применяемых в клеточной биологии и регенеративной медицине. Особое внимание авторы уделили факторам роста; описаны функциональные характеристики основных семейств этих полипептидов (факторы роста фибробластов, эпидермальные факторы роста, трансформирующие факторы роста, ростовые дифференцировочные, эпидермальные факторы роста, факторы роста эндотелиальных клеток, гемопоэтические ростовые факторы и др.). Отмечено, что одним из перспективных источников факторов роста является сыворотка (плазма) пуповинной крови. В обзоре приведены основные технологии получения пуповинной крови, а также систематизированы исследования, отражающие содержание ростовых факторов, цитокинов, экзосом и мРНК в пуповинной крови; описаны экспериментальные данные по применению сыворотки пуповинной крови в качестве добавки к культуральным средам для выращивания различных культур клеток животного происхождения. Сыворотка пуповинной крови человека, по сравнению с источниками животного происхождения, является доступным, безопасным продуктом, содержащим высокие уровни биологически активных молекул. Для широкого внедрения ее в качестве добавки к культуральным средам необходима разработка стандартов получения и тестирования этого продукта.

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