Cell and Tissue BiologyCell and Tissue BiologyЦитология0041-37713034-6061The Russian Academy of Sciences66953510.31857/S0041377123060056QPQWCJArticlesСтатьиGap Junction Protein Connexin-43 in Glial Cells of Rat Dorsal Root GanglionБелок щелевых контактов коннексин-43 в глиальных клетках чувствительного ганглия крысыKolosE. A.КолосЕ. А.koloselena1984@yandex.ruKorzhevskiiD. E.КоржевскийД. Э.koloselena1984@yandex.ruInstitute of Experimental MedicineИнститут экспериментальной медицины0111202365658359227022025Copyright ©; 2023, Е.А. Колос, Д.Э. КоржевскийCopyright ©; 2023, Е.А. Колос, Д.Э. Коржевский2023Е.А. Колос, Д.Э. КоржевскийЕ.А. Колос, Д.Э. Коржевскийhttps://vietnamjournal.ru/0041-3771/article/view/669535

The aim of this study was to assess the dynamics of distribution and localization of the gap junction protein connexin-43 (Cx43) in rat dorsal root ganglion (DRG) cells at different stages of postnatal ontogenesis to assess the morphological signs of age-related changes in intercellular interactions. The work was performed on Wistar rats at the age of 4 months and 18 months using immunohistochemical methods. Glial cells were detected using antibodies to glutamine synthetase, macrophages – using the antibodies to calcium-binding protein Iba-1. The paper describes the features of connexin-43 distribution in the spinal ganglion of young and old rats. It has been established that connexin-43-containing structures are identified mainly in satellite glial cells of young and aging animals. Sensitive neurons, as well as DRG macrophages of both groups of animals, do not show immunoreactivity. Analysis of age-related changes in intercellular contacts in rat DRG showed that plaques of connexin-43-containing protein channels that provide metabolic interaction of satellite cells in the spinal ganglia become more numerous with age. This fact may indicate the activation of the interaction between glial cells in the DRG of rats during aging.

Цель настоящего исследования состояла в изучении динамики распределения и локализации белка щелевых контактов коннексина-43 (Сx43) в клетках ганглия заднего корешка спинного мозга (dorsal root ganglion, DRG) крысы на разных этапах постнатального онтогенеза для оценки морфологических признаков возрастных изменений межклеточных взаимодействий. Работа выполнена на крысах Вистар в возрасте 4 и 18 мес с помощью иммуногистохимических методов. Глиальные клетки выявляли с применением антител к глутаминсинтетазе, макрофаги – с применением маркера Iba-1. Установлено, что коннексин-43-содержащие структуры идентифицируются преимущественно в сателлитных глиальных клетках молодых и стареющих животных. Чувствительные нейроны, а также макрофаги DRG крыс исследованных возрастных групп коннексин-43 не содержат. При анализе возрастных изменений межклеточных контактов в DRG крыс было установлено, что зоны, обогащенные коннексином-43, соответствующие бляшкам (plaques) белковых каналов, обеспечивающие метаболическое взаимодействие сателлитных клеток в ганглиях задних корешков спинного мозга, с возрастом становятся более многочисленными. Данный факт может свидетельствовать об активации взаимодействия между глиальными клетками в чувствительных узлах крыс при старении.

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