Cell and Tissue BiologyCell and Tissue BiologyЦитология0041-37713034-6061The Russian Academy of Sciences66949910.31857/S0041377124040035QDCCGYArticlesСтатьиResearch ArticleNew line of mesenchymal stem cell isolated from warton’s jelly of the umbical cord of male human donorНовая линия мезенхимных стволовых клеток, выделенная из вартонова студня пупочного канатика донора мужского полаKoltsovaA. M.КольцоваА. М.
Russian Federation
koltsova.am@mail.ruMusorinaA. S.МусоринаА. С.
Russian Federation
koltsova.am@mail.ruTurilovaV. I.ТуриловаВ. И.
Russian Federation
koltsova.am@mail.ruShatrovaA. N.ШатроваА. Н.
Russian Federation
koltsova.am@mail.ruYakovlevaT. K.ЯковлеваТ. К.
Russian Federation
koltsova.am@mail.ruPoljanskayaG. G.ПолянскаяГ. Г.
Russian Federation
gpolanskaya@gmail.comInstitute of Cytology, Russian Academy of SciencesИнститут цитологии РАН1507202466434135427022025Copyright ©; 2024, Russian Academy of SciencesCopyright ©; 2024, Российская академия наук2024Russian Academy of SciencesРоссийская академия наукhttps://vietnamjournal.ru/0041-3771/article/view/669499

A new non-immortalized fibroblast-like cell line, named MSCWJ-3, was generated and characterized. Characteristics during long-term cultivation (6–24 passages) confirm the status of MSCs. It is shown: 1) a gradual increase in the proportion of senescent cells during long-term cultivation; 2) a significant decrease in the proliferation index by the 24th passage; 3) preservation of the normal diploid karyotype of the man (46, XY) during the entire period of cultivation, trisomy for different autosomes in single cells, absence of structural chromosomal rearrangements; 4) a high proportion of cells carrying surface antigens characteristic of MSCs: CD44, CD73, CD90, CD105, HLA-ABC and a low proportion with antigens CD34, CD45 and HLA-DR over 24 passages. Cells of the MSCWJ-3 line are capable of differentiation in the osteogenic and adipogenic directions at early and late passages; differentiation in the chondrogenic direction is absent. In general, there are some differences with previously obtained lines isolated from the same source and are associated mainly with the degree of expression of a number of status characteristics.

Получена и охарактеризована новая неиммортализованная фибробластоподобная клеточная линия, названная MSCWJ-3. Характеристики в процессе длительного культивирования (6–24 пассажи), подтверждают статус МСК. Показано: 1) постепенное увеличение доли стареющих клеток в процессе длительного культивирования; 2) значительное снижение индекса пролиферации к 24-му пассажу; 3) сохранение нормального диплоидного кариотипа мужчины (46, XY) в процессе всего срока культивирования, трисомия по разным аутосомам в единичных клетках, отсутствие структурных хромосомных перестроек; 4) высокая доля клеток, несущих поверхностные антигены, характерные для МСК: CD44, CD73, CD90, CD105, HLA-ABC и низкая – с антигенами CD34, CD45 и HLA-DR на протяжении 24 пассажей. Клетки линии MSCWJ-3 способны дифференцироваться в остеогенном и адипогенном направлениях на ранних и поздних пассажах; дифференцировка в хондрогенном направлении отсутствует. В целом отмечаются некоторые различия с ранее полученными линиями, выделенными из этого же источника и связанные, в основном, со степенью выраженности ряда статусных характеристик.

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